Influenza and Other Respiratory Viruses Department, Pasteur Institute of Iran, Tehran, Iran.
Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran.
Virol J. 2017 Nov 9;14(1):218. doi: 10.1186/s12985-017-0884-4.
IFITM3 has been suggested to be associated with infection in some ethnic groups. Diabetes and hypercholesterolemia are also important clinical conditions that can predispose individuals to infection. The aim of this study was to investigate the association of rs12252 C polymorphism, BMI, diabetes, and hypercholesterolemia with mild flu in an Iranian population.
We conducted a case-control study, including 79 mild flu and 125 flu-negative individuals attending primary care centers of three provinces of Iran (i.e, Markazi, Semnan, and Zanjan). Pharyngeal swab specimens were collected from all participants, and were subjected to RNA and DNA extractions for Real-time PCR and PCR tests. All PCR products were then sequenced to find T/C polymorphisms in the rs12252 region. Data on demographic, anthropometric, and clinical variables were collected from participants' medical records available in the primary care centers. The data was analyzed using DNASIS (v. 2.5) and Stata (v.11) software.
All participants were of Fars ethnic background. The allele frequency for rs12252-C was found to be 9.49% among cases and 2.40% among controls. Carriers of the rs12252 C allele (CT + CC genotypes) showed 5.92 folds increase in the risk of mild flu comparing to the T allele homozygotes (P value: 0.007). We also found a significant positive association between rs12252 C allele heterozygote and mild flu (OR: 7.62, P value: 0.008), but not in C allele homozygote group (OR: 2.71, P value: 0.406). Similarly, we did not find a significant association between mild flu and BMI (OR: 1.06, P value: 0.087), diabetes (OR: 0.61, P value: 0.392), and hypercholesterolemia (OR: 0.50, P value: 0.393) in multivariable logistic regression.
This is the first study evaluating the association between rs12252 polymorphisms, diabetes, hypercholesterolemia, and BMI and susceptibility to mild flu in an Iranian population. Our results suggest a significant positive association between mild flu and rs12252 C allele heterozygous and carriage. Future replication of the strong association observed here between rs12252 C allele carriage and mild flu might candidate this polymorphism as a genetic marker for early screening of susceptibility to mild flu. Lack of significant association between C allele homozygous and mild flu, observed in this study, might be the result of small sample size in this group.
IR.PII.REC.1395.3.
IFITM3 已被认为与某些族群的感染有关。糖尿病和高胆固醇血症也是重要的临床状况，可能使个体易感染。本研究的目的是调查伊朗人群中 rs12252 C 多态性、BMI、糖尿病和高胆固醇血症与轻度流感的关联。
我们进行了病例对照研究，包括 79 名轻度流感和 125 名流感阴性个体，他们在伊朗三个省份（即马赞德兰、塞姆南和赞詹）的基层医疗中心就诊。从所有参与者中采集咽拭子标本，进行 RNA 和 DNA 提取，用于实时 PCR 和 PCR 检测。然后对所有 PCR 产物进行测序，以发现 rs12252 区域的 T/C 多态性。从基层医疗中心的参与者病历中收集人口统计学、人体测量学和临床变量的数据。使用 DNASIS（v. 2.5）和 Stata（v.11）软件分析数据。
所有参与者均为法尔斯族裔。病例组的 rs12252-C 等位基因频率为 9.49%，对照组为 2.40%。与 T 等位基因纯合子相比，携带 rs12252 C 等位基因（CT+CC 基因型）的个体患轻度流感的风险增加了 5.92 倍（P 值：0.007）。我们还发现 rs12252 C 等位基因杂合子与轻度流感之间存在显著正相关（OR：7.62，P 值：0.008），但 C 等位基因纯合子组无显著相关（OR：2.71，P 值：0.406）。同样，在多变量逻辑回归中，我们也没有发现 BMI（OR：1.06，P 值：0.087）、糖尿病（OR：0.61，P 值：0.392）和高胆固醇血症（OR：0.50，P 值：0.393）与轻度流感之间存在显著关联。
这是第一项评估伊朗人群中 rs12252 多态性、糖尿病、高胆固醇血症和 BMI 与轻度流感易感性之间关联的研究。我们的结果表明，轻度流感与 rs12252 C 等位基因杂合子和携带之间存在显著正相关。如果未来能够复制观察到的 rs12252 C 等位基因携带与轻度流感之间的强关联，那么该多态性可能成为早期筛查轻度流感易感性的遗传标志物。在本研究中，未观察到 C 等位基因纯合子与轻度流感之间存在显著关联，这可能是由于该组样本量较小所致。
IR.PII.REC.1395.3.
