蜘蛛（蜘蛛目，捕鸟蛛科）毒素VdTX-I的抗菌活性。
Antimicrobial activity of the toxin VdTX-I from the spider (Araneae, Theraphosidae).
作者信息
Sutti Rafael, Rosa Bruno Bezerra, Wunderlich Bettina, da Silva Junior Pedro Ismael, Rocha E Silva Thomaz Augusto Alves da
机构信息
Departamento de Ciências Fisiológicas-Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, Brasil.
Johns Hopkins University, Baltimore, MD, USA.
出版信息
Biochem Biophys Rep. 2015 Sep 28;4:324-328. doi: 10.1016/j.bbrep.2015.09.018. eCollection 2015 Dec.
BACKGROUND
Currently there is an urgent need to develop new classes of antimicrobial agents with different mechanisms of action from conventionally antibiotics used for the control of pathogenic microorganisms. The acylpolyamine called VdTX-I was isolated from the venom of the tarantula , and first described with activity as an antagonist of nicotinic cholinergic receptors. The main objective of this study was to investigate the antimicrobial activity found in the venom of the spider, with emphasis on the toxin VdTX-I.
METHODS
Antimicrobial assays were performed in 96 well plates culture against 14 micro-organisms (fungi, yeasts and bacteria), which were tested concentrations from 0.19 to 100 μM of VdTX-I. After qualitative analysis, dose-response curve assays were performed in bacterial kill curve using MTT reagent and hemolytic assay.
RESULTS
The antimicrobial activity of the VdTX-I toxin was observed in 12 tested species of , , and . The toxicity had a dose-response at 3.12 µM - 100 μM in , , and VdTX-I took about 5 min to inhibit bacterial growth, which was faster than streptomycin. The toxin showed no hemolytic activity between 0.19 and 100 μM. At 2.5 µg/mL of toxin it was observed no growth inhibition against a mammalian cell lineage.
CONCLUSIONS
The VdTX-I toxin has a significant antimicrobial activity, with broad spectrum, and is experimentally inert to mammalian blood cells.
GENERAL SIGNIFICANCE
This paper explores the antimicrobial potential of the spider toxin VdTX-I, which can provide a new model to design new antimicrobial drugs.
背景
目前迫切需要研发新型抗菌剂，其作用机制与用于控制病原微生物的传统抗生素不同。从狼蛛毒液中分离出一种名为VdTX-I的酰基多胺，最初将其描述为烟碱型胆碱能受体的拮抗剂。本研究的主要目的是研究蜘蛛毒液中的抗菌活性，重点是毒素VdTX-I。
方法
在96孔板培养中对14种微生物（真菌、酵母和细菌）进行抗菌试验，测试VdTX-I的浓度范围为0.19至100μM。经过定性分析后，使用MTT试剂进行细菌杀伤曲线的剂量反应曲线测定和溶血试验。
结果
在12种测试的[此处原文缺失具体微生物种类]中观察到VdTX-I毒素的抗菌活性。在[此处原文缺失具体细菌种类]中，毒性在3.12µM - 100μM呈现剂量反应。VdTX-I抑制细菌生长约需5分钟，比链霉素更快。该毒素在0.19至100μM之间未显示溶血活性。在2.5µg/mL的毒素浓度下，未观察到对哺乳动物细胞系的生长抑制作用。
结论
VdTX-I毒素具有显著的抗菌活性，抗菌谱广，并且对哺乳动物血细胞在实验上无活性。
一般意义
本文探索了蜘蛛毒素VdTX-I的抗菌潜力，可为设计新型抗菌药物提供新的模型。
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