Iarikov Dmitri D, Kargar Mehdi, Sahari Ali, Russel Lauren, Gause Katelyn T, Behkam Bahareh, Ducker William A
Department of Chemical Engineering, ‡Department of Mechanical Engineering, and §School of Biomedical Engineering and Sciences, Virginia Tech , Blacksburg, Virginia 24061, United States.
Biomacromolecules. 2014 Jan 13;15(1):169-76. doi: 10.1021/bm401440h. Epub 2013 Dec 16.
We investigated the antimicrobial properties of the cationic polymer polyallylamine (PA) when covalently bonded to glass. The objective was to obtain a robust attachment, yet still allow extension of the polymer chain into solution to enable interaction with the bacteria. The PA film displayed strong antimicrobial activity against Staphylococcus epidermidis , Staphylococcus aureus , and Pseudomonas aeruginosa , which includes both Gram-positive and Gram-negative bacteria. Glass surfaces were prepared by a straightforward two-step procedure of first functionalizing with epoxide groups using 3-glycidoxypropyltrimethoxy silane (GOPTS) and then exposing to PA so that the PA could bind via reaction of a fraction of its amine groups. The surfaces were characterized using X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy to verify the presence of the polymer on the surface, zeta potential measurements to estimate the surface charge of the films, and atomic force microscopy to determine the extension of the polymer chains into solution. Antimicrobial properties of these coatings were evaluated by spraying aqueous suspensions of bacteria on the functionalized glass slides, incubating them under agar, and counting the number of surviving cell colonies.
我们研究了共价键合到玻璃上的阳离子聚合物聚烯丙胺（PA）的抗菌性能。目的是实现牢固附着，同时仍使聚合物链延伸到溶液中以便与细菌相互作用。PA膜对表皮葡萄球菌、金黄色葡萄球菌和铜绿假单胞菌表现出强大的抗菌活性，这些细菌包括革兰氏阳性菌和革兰氏阴性菌。玻璃表面通过一个简单的两步程序制备，首先使用3-缩水甘油氧基丙基三甲氧基硅烷（GOPTS）用环氧基团进行功能化，然后暴露于PA，使PA能够通过其一部分胺基的反应进行结合。使用X射线光电子能谱和傅里叶变换红外光谱对表面进行表征，以验证表面上聚合物的存在，使用zeta电位测量来估计膜的表面电荷，并使用原子力显微镜来确定聚合物链向溶液中的延伸。通过将细菌的水悬浮液喷洒在功能化的载玻片上，在琼脂下孵育它们，并计数存活细胞集落的数量来评估这些涂层的抗菌性能。
