Oguntimein Gbekeloluwa B, Rodriguez Miguel, Dumitrache Alexandru, Shollenberger Todd, Decker Stephen R, Davison Brian H, Brown Steven D
Morgan State University, Baltimore, MD, USA.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
Biotechnol Lett. 2018 Feb;40(2):303-308. doi: 10.1007/s10529-017-2467-2. Epub 2017 Nov 9.
To develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential.
Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain.
A prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.
开发并制作一种高通量微孔板检测方法，以便在对联合生物加工潜力进行快速、经济高效的筛选中评估厌氧微生物和木质纤维素生物质。
嗜热栖热菌亲本Δhpt菌株将微晶纤维素分解为纤维二糖、葡萄糖，并产生乳酸、甲酸、乙酸和乙醇作为发酵产物，其滴度和比例与大规模发酵相似，证实了基于平板的方法适用于嗜热栖热菌的生长研究。与Δhpt菌株相比，在关键中心代谢途径中存在基因缺失的嗜热栖热菌LL1210菌株，在联合生物加工（CBP）平板检测中对微晶纤维素和柳枝稷发酵产生的乙醇滴度更高。
开发了一种原型微孔板检测系统，该系统将有助于对用于生物乙醇生产的新型木质纤维素生物质类型、遗传变体和新微生物菌株进行高通量生物勘探。
