松果体多肽复合物中肽AEDG的鉴定。

Identification of Peptide AEDG in the Polypeptide Complex of the Pineal Gland.

作者信息

Khavinson V Kh, Kopylov A T, Vaskovsky B V, Ryzhak G A, Lin'kova N S

机构信息

Research Centre St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia.

I. P. Pavlov Institute of Physiology of Russian Academy of Sciences, St. Petersburg, Russia.

出版信息

Bull Exp Biol Med. 2017 Nov;164(1):41-43. doi: 10.1007/s10517-017-3922-8. Epub 2017 Nov 9.

DOI:10.1007/s10517-017-3922-8

PMID:29124531

Abstract

The polypeptide complex of the epiphysis and the peptide AEDG, constructed on the basis of its amino acid analysis, exert similar biological effects. Both bioregulators normalize melatonin synthesis in the pineal gland, functioning of the brain, eye retina, cardiovascular, endocrine, and immune systems; they also act as antioxidants, stress-protectors, and geroprotectors. Within the epiphysis polypeptide complex, free amino acids (3.26%), dipeptides (23.19%), tripeptides (50.72%), tetrapeptides (22.10%), and pentapeptides (0.72%) were revealed by mass spectrometry and HPLC. Peptide AEDG was detected among the tetrapeptides of the epiphysis polypeptide complex by selective reaction monitoring method. The biological effects of the epiphysis polypeptide complex are determined by the effect of its component AEDG.

摘要

松果体的多肽复合物以及基于氨基酸分析构建的肽AEDG具有相似的生物学效应。这两种生物调节剂均可使松果体中的褪黑素合成、大脑、眼视网膜、心血管、内分泌和免疫系统的功能正常化；它们还具有抗氧化剂、应激保护剂和老年保护剂的作用。通过质谱分析和高效液相色谱法发现，松果体多肽复合物中含有游离氨基酸（3.26%）、二肽（23.19%）、三肽（50.72%）、四肽（22.10%）和五肽（0.72%）。通过选择性反应监测法在松果体多肽复合物的四肽中检测到了肽AEDG。松果体多肽复合物的生物学效应由其成分AEDG的作用决定。