Leinsoo A T, Shaskol'skii B L, Dement'eva E I, Gryadunov D A, Kubanov A A, Chestkov A V, Obraztsova O A, Shpilevaya M V, Deryabin D G
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
State Research Center of Dermatovenerology and Cosmetology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2017 Nov;164(1):54-60. doi: 10.1007/s10517-017-3925-5. Epub 2017 Nov 10.
We developed a multiplexed DNA microarray-based assay allowing identification of 12 causative agents of reproductive tract infections with the simultaneous detection of 47 genetic determinants of resistance to antimicrobial substances. The microarray was tested on 93 isolates of Neisseria gonorrhoeae, 32 isolates of Treponema pallidum and 29 samples of Ureaplasma spp./Mycoplasma spp. The N. gonorrhoeae isolates had multiple mutations in the penA, ponA, rpsJ, gyrA, parC, and mtrR genes; their prognostic value significantly increased when combinations of mutations were detected. In the analyzed T. pallidum isolates, single A2058G substitution in the 23S rRNA gene responsible for macrolide resistance was found. DNA sequences of Ureaplasma spp./Mycoplasma spp. were determined as wild type, which was not fully consistent with the results of analysis of their antimicrobial susceptibility.
我们开发了一种基于多重DNA微阵列的检测方法，可鉴定12种生殖道感染病原体，同时检测47种对抗菌物质耐药的遗传决定因素。该微阵列在93株淋病奈瑟菌、32株梅毒螺旋体和29份解脲脲原体/支原体样本上进行了测试。淋病奈瑟菌分离株在penA、ponA、rpsJ、gyrA、parC和mtrR基因中存在多个突变；当检测到突变组合时，它们的预后价值显著增加。在所分析的梅毒螺旋体分离株中，发现23S rRNA基因中导致大环内酯耐药的单个A2058G替换。解脲脲原体/支原体的DNA序列被确定为野生型，这与它们的药敏分析结果并不完全一致。
