Guangzhou Jinan Biomedicine Research and Development Center, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, People's Republic of China. Department of Biomedical Engineering, University of Memphis, Memphis, TN, United States of America.
Biomed Mater. 2017 Nov 10;13(1):015004. doi: 10.1088/1748-605X/aa853c.
The use of chitosan based nanofiber membranes in guided bone regeneration (GBR) is limited by its uncontrolled swelling and mechanical instability in aqueous environments. This paper describes the significantly improved stability and properties of surface butyrylated chitosan nanofiber (BCSNF) membranes that greatly enhance their potential in GBR. The BCSNF membranes exhibited an overall degree of substitution of 1.61, an average diameter of 99.3 ± 33.7 nm, and a 75% decrease in swelling with an approximate doubling in suture pull out strengths as compared to unmodified fibers in aqueous environment. In a five week phosphate-buffered saline-lysozyme degradation study, it was found that the remaining mass fraction of BCSNF membranes was 11.5% more than that of unmodified fibers. In vitro, the BCSNF membranes were found to support the adhesion and proliferation of fibroblasts and were cell occulusive. In vivo, the BCSNF membranes were found to significantly improve the regeneration of a rat calvarial critical size defect in a 12 week healing period and showed better barrier function than commercially available collagen membranes with little soft tissue penetration through the membranes. Taken together, these data provide strong scientific evidence for use of BCSNF membranes in GBR applications.
壳聚糖基纳米纤维膜在引导骨再生（GBR）中的应用受到其在水相环境中不可控溶胀和机械不稳定的限制。本文描述了经过表面丁酰化修饰的壳聚糖纳米纤维（BCSNF）膜的稳定性和性能得到了显著提高，这极大地增强了其在 GBR 中的应用潜力。BCSNF 膜的取代度为 1.61，平均直径为 99.3±33.7nm，在水相环境中的溶胀度降低了 75%，缝线拔出强度增加了约一倍。在为期五周的磷酸盐缓冲盐水-溶菌酶降解研究中，发现 BCSNF 膜的剩余质量分数比未修饰纤维多 11.5%。体外实验发现，BCSNF 膜支持成纤维细胞的黏附和增殖，并且具有细胞封闭性。体内实验发现，BCSNF 膜在 12 周的愈合期内显著改善了大鼠颅骨临界尺寸缺损的再生，并表现出比商业上可用的胶原膜更好的屏障功能，通过膜的软组织渗透很少。综上所述，这些数据为 BCSNF 膜在 GBR 应用中的使用提供了强有力的科学依据。
