新型重组多表位蛋白用于检测抗隐球菌抗体。
Novel recombinant multiepitope proteins for the detection of anti-Cryptococcus antibodies.
机构信息
Laboratório de Imunogenética e Biologia Molecular, Universidade Federal do Piauí, Campus Ministro Petrônio Portella Bloco SG-16, 64049-550, Teresina, Piauí, Brasil.
Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Parasitologia, 31279-910, Belo Horizonte, Minas Gerais, Brasil.
出版信息
Future Microbiol. 2018 Mar;13:429-436. doi: 10.2217/fmb-2017-0184. Epub 2017 Nov 10.
AIM
To produce and test recombinant multiepitope proteins as an alternative assay for the serological diagnosis of cryptococcosis.
MATERIALS & METHODS: Previously, synthetic peptides were used to detect anti-Cryptococcus antibodies, and in silico analyses showed that the union of peptides would improve the results. Here, the coding sequences of these peptides were assembled into synthetic genes. Four genes have been cloned and expressed in Escherichia coli, producing recombinant multiepitope proteins: proteins A, B, C and D.
RESULTS
All constructs yielded good results; however, protein D showed the best results, with a sensitivity of 88.57% and specificity of 100%.
CONCLUSION
The multiepitope proteins were shown to be potential antigens for the diagnosis of cryptococcosis in an attempt to detect anti-Cryptococcus antibodies.
目的
制备并测试重组多表位蛋白，作为隐球菌病血清学诊断的替代检测方法。
材料与方法
既往，我们使用合成肽来检测抗隐球菌抗体，而计算机分析表明肽的联合使用将改善检测结果。在此，我们将这些肽的编码序列组装到合成基因中。已经克隆并在大肠杆菌中表达了 4 个基因，产生了重组多表位蛋白：蛋白 A、B、C 和 D。
结果
所有构建体均获得了良好的结果；然而，蛋白 D 显示出最佳的结果，其敏感性为 88.57%，特异性为 100%。
结论
多表位蛋白被证明是潜在的隐球菌病诊断抗原，旨在检测抗隐球菌抗体。
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