Mayes Carrie, Seashols-Williams Sarah, Hughes-Stamm Sheree
Department of Forensic Science, College of Criminal Justice, Sam Houston State University, 1003 Bowers Blvd., Huntsville, TX 77340-2525, United States.
Department of Forensic Science, Virginia Commonwealth University, Box 843079, Richmond, VA 23284, United States.
Leg Med (Tokyo). 2018 Jan;30:1-4. doi: 10.1016/j.legalmed.2017.10.013. Epub 2017 Oct 31.
Body fluid identification (BFID) can provide crucial information during the course of an investigation. In recent years, microRNAs (miRNAs) have shown considerable body fluid specificity, are able to be co-extracted with DNA, and their small size (18-25 nucleotides) make them ideal for analyzing highly degraded forensic samples. In this study, we designed a preliminary 8-marker system for BFID including an endogenous reference gene (let-7g) to differentiate between venous blood (miR-451a and miR-142-3p), menstrual blood (miR-141-3p and miR-412-3p), semen (miR-891a and miR-10b), and saliva (miR-205) using a capillary electrophoresis approach. This panel uses a linear primer system in order to incorporate additional miRNA markers by forming a multiplex system. The miRNA system was able to distinguish between venous blood, menstrual blood, semen, and saliva using a rudimentary data interpretation strategy. All STR amplifications from co-extracted DNA yielded complete profiles from human identification purposes.
体液鉴定（BFID）在调查过程中可提供关键信息。近年来，微小RNA（miRNA）已显示出相当高的体液特异性，能够与DNA共同提取，并且其小尺寸（18 - 25个核苷酸）使其成为分析高度降解的法医样本的理想选择。在本研究中，我们设计了一个用于BFID的初步8标记系统，包括一个内源性参考基因（let - 7g），以使用毛细管电泳方法区分静脉血（miR - 451a和miR - 142 - 3p）、月经血（miR - 141 - 3p和miR - 412 - 3p）、精液（miR - 891a和miR - 10b）以及唾液（miR - 205）。该面板使用线性引物系统，以便通过形成多重系统纳入额外的miRNA标记。该miRNA系统能够使用基本的数据解释策略区分静脉血、月经血、精液和唾液。从共同提取的DNA进行的所有STR扩增均产生了用于人类身份鉴定目的的完整图谱。
