Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki 852-8521, Japan.
Comp Biochem Physiol Part D Genomics Proteomics. 2018 Mar;25:26-33. doi: 10.1016/j.cbd.2017.10.003. Epub 2017 Oct 17.
While marine invertebrate cytochrome P450 (CYP) genes and their roles in detoxification mechanisms have been studied, little information is available regarding freshwater rotifer CYPs and their functions. Here, we used genomic sequences and RNA-seq databases to identify 31 CYP genes in the freshwater rotifer Brachionus calyciflorus. The 31 Bc-CYP genes with a few tandem duplications were clustered into CYP 2, 3, 4, mitochondrial, and 46 clans with two marine rotifers Brachionus plicatilis and Brachionus koreanus. To understand the molecular responses of these 31 Bc-CYP genes, we also examined their expression patterns in response to benzo[α]pyrene (B[α]P). Three Bc-CYP genes (Bc-CYP3044B3, Bc-CYP3049B4, Bc-CYP3049B6) were significantly upregulated (P<0.05) in response to B[α]P, suggesting that these CYP genes can be involved in detoxification in response to B[α]P exposure. These genes might be useful as biomarkers of B[α]P exposure in B. calyciflorus. Overall, our findings expand the repertoire of known CYPs and shed light on their potential roles in xenobiotic detoxification in rotifers.
虽然已经研究了海洋无脊椎动物细胞色素 P450（CYP）基因及其在解毒机制中的作用，但关于淡水轮虫 CYP 及其功能的信息却很少。在这里，我们使用基因组序列和 RNA-seq 数据库鉴定了淡水轮虫褶皱臂尾轮虫中的 31 个 CYP 基因。这 31 个 Bc-CYP 基因存在一些串联重复，聚类为 CYP2、3、4、线粒体和 46 族，与两种海洋轮虫褶皱臂尾轮虫和秀丽白虾轮虫共有。为了了解这 31 个 Bc-CYP 基因的分子反应，我们还检查了它们在苯并[a]芘（B[α]P）响应下的表达模式。三个 Bc-CYP 基因（Bc-CYP3044B3、Bc-CYP3049B4、Bc-CYP3049B6）在响应 B[α]P 时显著上调（P<0.05），表明这些 CYP 基因可能参与 B[α]P 暴露的解毒。这些基因可能作为褶皱臂尾轮虫 B[α]P 暴露的生物标志物。总的来说，我们的发现扩展了已知 CYP 的种类，并阐明了它们在轮虫中外源物质解毒中的潜在作用。
