Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent 9000, Belgium.
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2018 Mar;25:9-18. doi: 10.1016/j.cbd.2017.10.004. Epub 2017 Oct 18.
Complex structural and biochemical processes take place in insect testis maturation, but the mechanisms by which functional proteins induce and regulate male fertility are unknown. Proteomics has been widely used to identify functional proteins involved in such complex physiological processes. In this study, we performed a label-free based quantitative proteomic analysis of developing testis of Bactrocera dorsalis with the aim of shedding light on spermatogenesis, sperm formation and also tissue development. A total of 1912 reliable proteins were identified, including 1589, 1705 and 1723 proteins in 1-, 5- and 9-d-old testis, respectively. Most of these proteins (76.68%) were identified by two to ten peptides, and the mean number of peptides was 7.46 with an average sequence coverage of 30.71%. Quantitative proteomic analysis showed that there were 141 and 111 proteins which were abundant in the 5- and 9-d-old testis, respectively. The Gene Ontology (GO) and Clusters of Orthologous Groups (COG) analyses showed that many of these proteins function in cellular and metabolic processes, including binding and catalytic activities in molecular function analysis, and energy production, cell division, and cell motility in COG analysis. Potential functional proteins, such as heat shock proteins, glutathione S-transferase, transferrin, metalloproteinase and energy metabolism-related proteins, were found to be abundant in the intermediate and sexual maturity stages of testis. The findings in this study help us to better understand the molecular mechanisms behind testis development and spermatogenesis, which is essential for manipulating male fertility for ecological regulation and potential control of this species.
昆虫睾丸成熟过程中发生复杂的结构和生化过程，但功能蛋白诱导和调节雄性生育力的机制尚不清楚。蛋白质组学已广泛用于鉴定参与这种复杂生理过程的功能蛋白。在这项研究中，我们对黑腹果蝇发育中的睾丸进行了无标记的定量蛋白质组学分析，旨在阐明精子发生、精子形成以及组织发育。共鉴定出 1912 种可靠的蛋白质，包括 1 日龄、5 日龄和 9 日龄睾丸中的 1589、1705 和 1723 种蛋白质。这些蛋白质中的大多数（76.68%）通过 2 到 10 个肽段被鉴定出来，平均肽段数为 7.46，平均序列覆盖率为 30.71%。定量蛋白质组学分析表明，有 141 种和 111 种蛋白质在 5 日龄和 9 日龄睾丸中丰富。基因本体论（GO）和直系同源群（COG）分析表明，这些蛋白质中的许多在细胞和代谢过程中发挥作用，包括分子功能分析中的结合和催化活性，以及 COG 分析中的能量产生、细胞分裂和细胞运动。发现热休克蛋白、谷胱甘肽 S-转移酶、转铁蛋白、金属蛋白酶和与能量代谢相关的蛋白质等潜在功能蛋白在睾丸的中期和性成熟阶段丰富。本研究的结果有助于我们更好地理解睾丸发育和精子发生的分子机制，这对于操纵雄性生育力以进行生态调节和对该物种的潜在控制至关重要。
