Wiltbank M C, Gallagher K P, Dysko R C, Keyes P L
Department of Physiology, University of Michigan, Ann Arbor 48109-0622.
Endocrinology. 1989 Feb;124(2):605-11. doi: 10.1210/endo-124-2-605.
We tested the hypothesis that estrogen and hCG can modify blood flow in the rabbit corpus luteum. Radioactively labeled microspheres were used to measure luteal blood flow in pseudopregnant rabbits in which estrogen had been withdrawn to initiate premature luteal regression and in pseudopregnant rabbits injected with hCG. Removal of estradiol-filled Silastic capsules on day 10 of pseudopregnancy caused an 80% decrease in the serum progesterone concentration within 24 h. Despite the decline in progesterone secretion, luteal blood flow remained at very high levels and was not different from that in control rabbits treated continuously with estradiol. Replacement of estradiol-filled capsules for 3 h did not change the high rate of blood flow to the corpus luteum, but blood flow in the uterus, vagina, and ovarian stroma was increased. The injection of hCG (10 IU, iv) on day 10 of pseudopregnancy caused a 3-fold increase in blood flow to the nonluteal portion of the ovary and a 3-fold increase in the serum progesterone concentration, but luteal blood flow did not change. We conclude that the acute actions of estradiol or hCG in the rabbit corpus luteum are not mediated by changes in luteal blood flow. Further, the results suggest that the luteal vasculature is regulated differently from the vasculature of other estrogen-responsive tissues and that blood flow in the nonluteal tissues of the ovary can be regulated independently of blood flow in the corpus luteum.
我们检验了雌激素和人绒毛膜促性腺激素（hCG）可改变兔黄体血流的假说。使用放射性标记的微球来测量假孕兔的黄体血流，其中一些假孕兔已撤除雌激素以引发黄体过早退化，另一些则注射了hCG。在假孕第10天移除填充雌二醇的硅橡胶胶囊，导致血清孕酮浓度在24小时内下降80%。尽管孕酮分泌减少，但黄体血流仍维持在非常高的水平，与持续接受雌二醇治疗的对照兔并无差异。重新放置填充雌二醇的胶囊3小时，并未改变流向黄体的高血流速率，但子宫、阴道和卵巢基质的血流增加。在假孕第10天静脉注射hCG（10 IU），导致卵巢非黄体部分的血流增加3倍，血清孕酮浓度也增加3倍，但黄体血流未改变。我们得出结论，雌二醇或hCG对兔黄体的急性作用并非由黄体血流变化介导。此外，结果表明黄体血管系统的调节方式与其他雌激素反应性组织的血管系统不同，且卵巢非黄体组织中的血流可独立于黄体血流进行调节。
