Moutos D, Schryer B, Hurn P, Dharmarajan A M
Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
J Reprod Fertil. 1995 Mar;103(2):357-62. doi: 10.1530/jrf.0.1030357.
The effect of exogenous oestrogen on blood flow and quantitative histology of the corpus luteum were studied in pseudopregnant rabbits. Pseudopregnancy was induced with human chorionic gonadotrophin and an oestradiol capsule was implanted on day 0 of pseudopregnancy. Regression of the corpus luteum was initiated in the mid-luteal phase by removal of the capsule. Thirty-two New Zealand white rabbits were randomly allocated to four groups (eight rabbits per group) for studies of blood flow--control: oestradiol capsule not removed: -24 h: oestradiol capsule removed 24 h before blood flow measurements; -48 h: oestradiol capsule removed for 48 h; and -48 h/+48 h: oestradiol capsule removed for 48 h and then reimplanted for an additional 48 h. All blood flow studies were performed on day 11 of pseudopregnancy with radioactive microspheres. Quantitative histology was performed on a separate group of 16 rabbits allocated to the same groups as above (four rabbits per group). Blood flow to the corpus luteum was unchanged in the -24 h group, but declined by 44% in the -48 h group. Blood flow returned to control values in the -48 h/+48 h group. Quantitative histology revealed no changes in the -24 h group, but a significant decrease in volume of the corpus luteum and volume of the luteal cell cytoplasm in the -48 h group. Corpus luteum volume was fully restored and luteal cell cytoplasm volume was partially restored in the -48 h/+48 h group. In contrast, the number of luteal cells, vessel space volume and capillary surface area did not change with oestrogen withdrawal.(ABSTRACT TRUNCATED AT 250 WORDS)
在假孕兔中研究了外源性雌激素对黄体血流及定量组织学的影响。用人绒毛膜促性腺激素诱导假孕，并在假孕第0天植入雌二醇胶囊。在黄体中期通过移除胶囊启动黄体退化。32只新西兰白兔被随机分为四组（每组8只兔）进行血流研究——对照组：未移除雌二醇胶囊；-24小时组：在血流测量前24小时移除雌二醇胶囊；-48小时组：移除雌二醇胶囊48小时；以及-48小时/+48小时组：移除雌二醇胶囊48小时，然后再植入48小时。所有血流研究均在假孕第11天用放射性微球进行。对另一组16只兔子进行定量组织学研究，这些兔子被分配到上述相同的组中（每组4只兔）。-24小时组黄体血流无变化，但-48小时组下降了44%。-48小时/+48小时组血流恢复到对照值。定量组织学显示，-24小时组无变化，但-48小时组黄体体积和黄体细胞胞质体积显著减小。-48小时/+48小时组黄体体积完全恢复，黄体细胞胞质体积部分恢复。相比之下，黄体细胞数量、血管腔体积和毛细血管表面积并未随雌激素撤除而改变。（摘要截短至250字）
