Laboratory of Nutrition Physiology and Feed Formulation, Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
Laboratory of Nutrition Physiology and Feed Formulation, Fishery College, Huazhong Agricultural University, Wuhan 430070, China; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China.
Ecotoxicol Environ Saf. 2018 Feb;148:578-584. doi: 10.1016/j.ecoenv.2017.10.064. Epub 2017 Nov 9.
The present study explored the influence of Zn addition in the water on Zn transport and lipid metabolism of two intestinal regions in goby Synechogobius hasta. Zn contents in water were 0.004 (control), 0.181 and 0.361mg Zn L, respectively. The experiment lasted for 28 days. TG and Zn contents, mRNA contents of genes of Zn transport and lipid metabolism, and enzyme activity from anterior and mid-intestine tissues were analyzed. In anterior intestine, Zn addition in the water increased Zn contents, and mRNA concentrations of ZIP4, ZIP5, ATGL, PPARα, ZNF202 and KLF7, decreased TG contents, 6PGD and G6PD activities, and mRNA contents of 6PGD, G6PD, FAS, PPARγ, ICDH and KLF4. In mid-intestine tissue, the highest Zn and TG contents were observed for 0.18mg Zn/l group, in parallel with the highest expressions of ZnT1, ZIP4, ZIP5, 6PGD, FAS, ICDH, PPARγ, PPARα, ZNF202, KLF4 and KLF7, and with the highest FAS, 6PGD and G6PD activities. Thus, in the anterior intestine, Zn addition increased lipolysis and decreased lipogenesis, and accordingly reduced TG content. However, the highest mid-intestinal TG content in 0.18mg Zn/l group was due to the up-regulated lipogenesis. Although lipolysis was also increased, the incremental lipid synthesis was enough to compensate for lipid degradation, which led TG accumulation. Our results, for the first time, show an anterior/mid functional regionalization of the intestine in lipid metabolism and Zn transport of S. hasta following Zn exposure.
本研究探讨了水中锌添加对两种肠道区域的鲫鱼 Synechogobius hasta 锌转运和脂质代谢的影响。水的锌含量分别为 0.004（对照）、0.181 和 0.361mg Zn/L。实验持续了 28 天。分析了前肠和中肠组织中的 TG 和锌含量、锌转运和脂质代谢基因的 mRNA 含量以及酶活性。在前肠中，水中添加锌增加了锌含量和 ZIP4、ZIP5、ATGL、PPARα、ZNF202 和 KLF7 的 mRNA 浓度，降低了 TG 含量、6PGD 和 G6PD 活性以及 6PGD、G6PD、FAS、PPARγ、ICDH 和 KLF4 的 mRNA 含量。在中肠组织中，0.18mg Zn/L 组观察到最高的 Zn 和 TG 含量，与 ZnT1、ZIP4、ZIP5、6PGD、FAS、ICDH、PPARγ、PPARα、ZNF202、KLF4 和 KLF7 的最高表达以及 FAS、6PGD 和 G6PD 活性最高相对应。因此，在前肠中，锌添加增加了脂肪分解，减少了脂肪生成，从而降低了 TG 含量。然而，在 0.18mg Zn/L 组中，中肠 TG 含量最高是由于上调的脂肪生成。尽管脂肪分解也增加了，但增量的脂质合成足以补偿脂质降解，导致 TG 积累。我们的研究结果首次表明，在锌暴露后，鲫鱼 S. hasta 的肠道在脂质代谢和锌转运方面存在前/中功能分区化。
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