Fe reduced hepatic lipid deposition in Synechogobius hasta exposed to waterborne Cu.

Recent evidences suggested that Fe influenced Cu metabolism in vertebrates. The present study was conducted to test the hypothesis that Fe could alleviate Cu-induced change of lipid deposition in the fish species. Synechogobius hasta were exposed to 0, 0.606 and 1.212μM Cu, in combination with 0 and 1.128μM Fe, respectively. Sampling occurred on day 28 and day 56, respectively. Growth performance, hepatic lipid deposition, Fe and Cu level, and activities and mRNA expression of enzymes and genes involved in lipid metabolism were analyzed. Fe addition in water improved survival in S. hasta exposed to the highest waterborne Cu concentration on day 56. Fe addition also increased hepatic Fe content both at day 28 and day 56, and reduced hepatic Cu content. Fe exposure tended to reduce the activities and mRNA expressions of lipogenic enzymes and genes (G6PD and FAS), and up-regulated the mRNA expression of ATGL. With the same Cu concentration, Fe addition tended to down-regulate mRNA levels of SREBP-1 and PPARγ, and up-regulate PPARα mRNA level on day 28. However, on day 56, the mRNA levels of SREBP-1, PPARγ and PPARα are very variable and not related with waterborne Fe addition. Some correlative relationship was observed between the mRNA of transcriptional factors, and the activities of enzymes and the mRNA expression of genes encoding them, implying their transcription regulation of these enzymatic genes by transcriptional factors after Fe addition. Overall, Fe addition mitigated Cu-induced changes of lipid deposition in fish by down-regulation of lipogenesis and up-regulation of lipolysis. Different response patterns of these enzyme activities and gene expressions in the liver of S. hasta following waterborne Fe exposure indicated that Fe effects on Cu-induced change of lipid metabolism are time-dependent.