Laboratory of Molecular Psychiatry, Center for Genes and Behavior, Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut.
Laboratory of Molecular Psychiatry, Center for Genes and Behavior, Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut.
Biol Psychiatry. 2018 May 15;83(10):840-848. doi: 10.1016/j.biopsych.2017.09.023. Epub 2017 Oct 4.
Posttraumatic stress disorder (PTSD) is a debilitating psychiatric disorder with a lifetime prevalence of nearly 8% in the general population. While the underlying molecular and cellular mechanisms of PTSD remain unknown, recent studies indicate that PTSD is associated with aberrant gene expression in brain as well as peripheral blood cells. The advent of next-generation sequencing technologies will allow us to elucidate the gene expression changes occurring in both brain and blood of patients with PTSD. RNA sequencing allows for analysis of the amount of transcript being made as well as alternative splicing, novel transcript identification, microRNA, and noncoding RNA quantification. Here we provide an overview of the different types of transcriptomic technologies as well as the gene expression studies performed in human peripheral blood and animal models of PTSD, and review the human PTSD postmortem brain gene profiling studies performed to date.
创伤后应激障碍（PTSD）是一种使人虚弱的精神疾病，在普通人群中的终身患病率接近 8%。虽然 PTSD 的潜在分子和细胞机制尚不清楚，但最近的研究表明，PTSD 与大脑和外周血细胞中的异常基因表达有关。下一代测序技术的出现将使我们能够阐明 PTSD 患者大脑和血液中发生的基因表达变化。RNA 测序可分析转录产物的数量以及选择性剪接、新转录本鉴定、microRNA 和非编码 RNA 的定量。本文提供了不同类型的转录组学技术概述，以及在人类外周血和 PTSD 动物模型中进行的基因表达研究，并回顾了迄今为止对人类 PTSD 尸检大脑基因图谱的研究。
