Shi Min, Nguyen Phuong, Jevremovic Dragan
Division of Hematopathology, Mayo Clinic, 200 1st Street Southwest, Hilton 8, Rochester, MN 55905, USA.
Division of Hematopathology, Mayo Clinic, 200 1st Street Southwest, Hilton 8, Rochester, MN 55905, USA.
Clin Lab Med. 2017 Dec;37(4):803-819. doi: 10.1016/j.cll.2017.07.006. Epub 2017 Sep 20.
Flow cytometry immunophenotyping of the hematopoietic cells from the bone marrow can help with diagnosis, prognosis, and therapy of chronic myeloid neoplasms. Unlike with B-cell neoplasms, there is no simple phenotypic test to substitute for clonality. Therefore, antigen panels to evaluate myeloid neoplasms are larger, and the gating strategies more complex than for lymphoid neoplasms. The number of phenotypic abnormalities in hematopoietic cells correlates with disease severity and cytogenetic complexity, and can be integrated into a scoring system for diagnostic and prognostic purposes. However, flow cytometry remains only an adjunct diagnostic modality.
对骨髓造血细胞进行流式细胞术免疫表型分析有助于慢性髓系肿瘤的诊断、预后评估及治疗。与B细胞肿瘤不同，没有简单的表型检测方法可替代克隆性检测。因此，评估髓系肿瘤的抗原组合比评估淋巴系肿瘤的更大，且设门策略也更复杂。造血细胞中表型异常的数量与疾病严重程度及细胞遗传学复杂性相关，可整合到一个评分系统中用于诊断和预后评估。然而，流式细胞术仍然只是一种辅助诊断手段。
