1,2-二油酰基-3-三甲铵丙烷（DOTAP）在β-乳球蛋白聚集中的作用。

Role of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) in β-lactoglobulin aggregation.

机构信息

Department of Physics, Science of College, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Chem Phys Lipids. 2018 Jan;210:76-81. doi: 10.1016/j.chemphyslip.2017.11.007. Epub 2017 Nov 8.

DOI:10.1016/j.chemphyslip.2017.11.007

Abstract

Protein aggregation is a prevalent phenomenon. It is important to study protein aggregation under different solution conditions. In this study, using 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence spectra, we investigated the critical micelle concentration (CMC) of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). We also studied the effects of DOTAP on amyloid aggregation of β-lactoglobulin using intrinsic fluorescence spectra, circular dichroism spectra, thioflavin T fluorescence, a Congo red binding assay and transmission electron microscopy. We observed that DOTAP had a dual role on β-lactoglobulin amyloid aggregation. DOTAP inhibited the amyloid aggregation below the CMC, while it had the opposite effect above the CMC. Moreover, the results of transmission electron microscopy showed that spherical aggregates were formed above the CMC. These results led us to conclude that cationic lipids could be used as modulators of protein self-assembly.

摘要

蛋白质聚集是一种普遍存在的现象。研究不同溶液条件下的蛋白质聚集非常重要。在本研究中，我们使用 1-苯胺基萘-8-磺酸（ANS）荧光光谱法研究了 1,2-二油酰基-3-三甲铵丙烷（DOTAP）的临界胶束浓度（CMC）。我们还使用内源荧光光谱法、圆二色光谱法、硫黄素 T 荧光法、刚果红结合测定法和透射电子显微镜研究了 DOTAP 对β-乳球蛋白淀粉样聚集的影响。我们观察到 DOTAP 对β-乳球蛋白淀粉样聚集具有双重作用。DOTAP 在 CMC 以下抑制淀粉样聚集，而在 CMC 以上则有相反的效果。此外，透射电子显微镜的结果表明，在 CMC 以上形成了球形聚集体。这些结果表明，阳离子脂质可以作为蛋白质自组装的调节剂。