Department of Biological Systems Engineering, Virginia Tech, 1230 Washington St. SW, Blacksburg, VA 24061, USA.
Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA.
J Virol Methods. 2018 Mar;253:61-69. doi: 10.1016/j.jviromet.2017.11.003. Epub 2017 Nov 9.
Porcine epidemic diarrhea Virus (PEDV) is the causative agent of porcine epidemic diarrhea, which is a devastating viral disease and causes severe economic loss to the swine industry. Current vaccine options for PEDV include modified live viruses and killed live viruses. Though these vaccines have shown efficacy, some have side effects including viral shedding. This report details an E. coli based expression and purification process of multiple vaccine candidates for PEDV using Hepatitis B Core Antigen (HBcAg) as a backbone protein. Short linear peptide sequences from PEDV were inserted into the immunodominant region of HBcAg in a novel recombinant vaccine design against PEDV. These peptide sequences were successfully inserted individually as well as all together in a multivalent strategy. Each vaccine candidate was tested in vivo in an intranasal as well as an intraperitoneal administration. Although each candidate was able to elicit a strong immunogenic response specific for the inserted peptide sequences, only two out of five of the test candidates demonstrated an ability to elicit an immune response capable of virus neutralization when delivered via intraperitoneal administration in mice.
猪流行性腹泻病毒（PEDV）是猪流行性腹泻的病原体，它是一种具有破坏性的病毒性疾病，给养猪业造成了严重的经济损失。目前用于预防 PEDV 的疫苗包括减毒活疫苗和灭活活疫苗。尽管这些疫苗已显示出一定的效果，但其中一些疫苗具有副作用，包括病毒脱落。本报告详细介绍了一种基于大肠杆菌的表达和纯化工艺，用于制备多个猪流行性腹泻病毒疫苗候选物，这些候选物以乙肝核心抗原（HBcAg）为骨架蛋白。在针对 PEDV 的新型重组疫苗设计中，将来自 PEDV 的短线性肽序列插入 HBcAg 的免疫优势区域。这些肽序列成功地单独插入，也可以以多价策略一起插入。每个候选疫苗都在体内通过鼻腔内和腹腔内给药进行了测试。尽管每个候选疫苗都能诱导针对插入肽序列的强烈免疫应答，但在腹腔内给药时，只有 5 个测试候选疫苗中的 2 个能够诱导出具有中和病毒能力的免疫应答。
