Choi Nakyung, Kim Heejin, Kim Byung Hee, Lee Junsoo, Kim In-Hwan
Department of Integrated Biomedical and Life Science, Graduate School, Korea University.
Department of Public Health Sciences, Graduate School, Korea University.
J Oleo Sci. 2017 Dec 1;66(12):1329-1335. doi: 10.5650/jos.ess17070. Epub 2017 Nov 13.
The synthesis of phytosteryl ester via the lipase-catalyzed esterification of phytosterol with fatty acid from echium oil was performed in a recirculating packed bed reactor. A commercial lipase from Candida rugosa was immobilized and then used to optimize the reaction conditions for the synthesis of the phytosteryl ester. The parameters investigated were temperature, molar ratio of phytosterol to fatty acid, retention time, and amount of solvent. The optimum conditions were determined as the temperature of 40℃, the molar ratio of 1:3 (phytosterol to fatty acid), the retention time of 3 min, and the solvent amount of 40 mL. The maximum conversion of phytosteryl ester was ca. 90 mol% under the optimum conditions. The major fatty acid in phytosteryl ester synthesized was α-linolenic acid (ca. 38 mol%).
通过脂肪酶催化植物甾醇与来自奇亚籽油的脂肪酸进行酯化反应合成植物甾醇酯，该反应在循环填充床反应器中进行。将来自皱褶假丝酵母的商业脂肪酶固定化，然后用于优化植物甾醇酯合成的反应条件。研究的参数包括温度、植物甾醇与脂肪酸的摩尔比、保留时间和溶剂量。确定的最佳条件为温度40℃、摩尔比1:3（植物甾醇与脂肪酸）、保留时间3分钟和溶剂量40 mL。在最佳条件下，植物甾醇酯的最大转化率约为90 mol%。合成的植物甾醇酯中的主要脂肪酸是α-亚麻酸（约38 mol%）。
