Department of Food and Nutrition, Korea University , Seoul 136-703, Republic of Korea.
J Agric Food Chem. 2013 Sep 18;61(37):8934-40. doi: 10.1021/jf402953n. Epub 2013 Sep 6.
Phytosteryl ester synthesized with pinolenic acid (PLA) from pine nut oil is expected to have features of both phytosterol and PLA. In this study, lipase from Candida rugosa (CRL) was immobilized and then used to optimize conditions for synthesis of phytosteryl ester containing PLA. Lewatit VP OC 1600, a macroporous hydrophobic resin, was selected as the best carrier, and the optimum condition for the immobilization of CRL was established. With immobilized CRL prepared, synthesis of phytosteryl ester with fatty acid from pine nut oil was carried out. Parameters investigated were temperature, molar ratio (phytosterol to fatty acid), enzyme loading, and vacuum. Optimum conditions for synthesis of phytosteryl ester were a temperature of 60 °C, molar ratio of 1:4, enzyme loading of 10% (based on the total weight of the substrate), and pressure of 80 kPa. The maximum conversion of phytosteryl ester was ca. 93 mol % at the optimum condition.
用松籽油中的松烯酸（PLA）合成的植物甾醇酯有望兼具植物甾醇和 PLA 的特性。在本研究中，固定化来自 Candida rugosa（CRL）的脂肪酶，然后用于优化含有 PLA 的植物甾醇酯合成的条件。Lewatit VP OC 1600 是一种大孔疏水树脂，被选为最佳载体，并确定了 CRL 固定化的最佳条件。使用制备的固定化 CRL，进行了从松籽油中的脂肪酸合成植物甾醇酯的实验。考察的参数为温度、摩尔比（植物甾醇与脂肪酸）、酶负载和真空度。合成植物甾醇酯的最佳条件为温度 60°C、摩尔比 1:4、酶负载 10%（基于底物总重量）、压力 80 kPa。在最佳条件下，植物甾醇酯的最大转化率约为 93 mol%。
