College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
Cell Tissue Res. 2018 Apr;372(1):23-31. doi: 10.1007/s00441-017-2707-8. Epub 2017 Nov 13.
In mammalian developing embryonic cortex, projection neurons migrate from the ventricular zone to the cortical plate, guided by radial glial cells with a transformation between bipolar and multipolar morphology. Previous studies have demonstrated that the PI3K-Akt-mTOR signal plays a critical role in brain development. However, the function of P85 in cortical development is still unclear. In the present study, we found that overexpression of P85 impaired cortical neuronal migration. Using in utero electroporation, we revealed that the length of the leading process in P85 overexpressed neurons became shorter than that in the control group but with more branches. Using markers for new-born neurons, we further found that overexpression of P85 did not affect the ultimate fate of these cortical neurons. These findings indicated that the P85 subunit plays an essential role in neuronal migration and neuronal morphology during mouse corticogenesis.
在哺乳动物发育中的胚胎皮层中，投射神经元在放射状胶质细胞的引导下从脑室区迁移到皮层板，其形态在双极和多极之间转换。先前的研究表明，PI3K-Akt-mTOR 信号在大脑发育中起着关键作用。然而，P85 在皮层发育中的功能尚不清楚。在本研究中，我们发现 P85 的过表达会损害皮质神经元的迁移。通过在体电穿孔，我们揭示了 P85 过表达神经元的突起的长度比对照组更短，但分支更多。使用标记新出生的神经元，我们进一步发现 P85 的过表达并不影响这些皮质神经元的最终命运。这些发现表明，P85 亚基在小鼠皮质发生过程中神经元迁移和神经元形态中发挥着重要作用。
