通过冷冻切片上的原位杂交-免疫电镜检测mRNA及相关分子

Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections.

作者信息

Rabouille Catherine

机构信息

Hubrecht Institute of the KNAW/University Medical Centre Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.

Department of Cell Biology, University Medical Center Utrecht, Utrecht, The Netherlands.

出版信息

Methods Mol Biol. 2018;1649:177-186. doi: 10.1007/978-1-4939-7213-5_11.

DOI:10.1007/978-1-4939-7213-5_11

PMID:29130197

Abstract

The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.

摘要

使用标记的RNA探针直接与化学固定组织的冷冻切片杂交，随后用特异性抗体和金偶联物进行标记检测，这构成了我们所开发并成功用于检测果蝇卵母细胞中内源性gurken和bicoid mRNA的原位杂交（ISH）-免疫电子显微镜（IEM）方法的核心。