Smith Malcolm D, Ahern Michael, Coleman Mark
Rheumatology Unit, Repatriation General Hospital, Daw Park, Adelaide, Australia.
Methods Mol Biol. 2006;326:235-45. doi: 10.1385/1-59745-007-3:235.
This chapter explores the combination of a nonradioactive in situ hybridization technique to detect mRNA with an imunnohistochemical labeling technique for use in formalin-fixed, paraffin-embedded, or frozen tissue sections. This technique allows the combination of detecting mRNA by in situ hybridization with immunohistochemical detection of a protein product or a cell surface marker without using any radioactive procedures. This technique is ideal for use on tissue sections when the aim is to identify which cells are producing a secreted protein product, such as a cytokine.
本章探讨了一种非放射性原位杂交技术与免疫组织化学标记技术的结合，用于福尔马林固定、石蜡包埋或冷冻组织切片，以检测mRNA。该技术允许通过原位杂交检测mRNA与蛋白质产物或细胞表面标志物的免疫组织化学检测相结合，而无需使用任何放射性程序。当目的是识别哪些细胞正在产生分泌性蛋白质产物（如细胞因子）时，该技术非常适合用于组织切片。
