无需核酸纯化的直接mRNA检测的杂交链式反应

Hybridization Chain Reaction for Direct mRNA Detection Without Nucleic Acid Purification.

作者信息

Xu Yao, Zheng Zhi

机构信息

Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, #5 Dong Dan San Tiao, Beijing, 100005, China.

出版信息

Methods Mol Biol. 2018;1649:187-196. doi: 10.1007/978-1-4939-7213-5_12.

DOI:10.1007/978-1-4939-7213-5_12

PMID:29130198

Abstract

Hybridization chain reaction (HCR) provides a feasible solution for nucleic acid detection without target amplification. By highly specific sandwich hybridization, target RNA can be directly captured onto solid support and detected using HCR with fluorescent dyes. Here, we describe a novel method for malaria RNA detection based on sandwich hybridization and two-dimensional HCR, without involving nucleic acid purification or any enzymatic reaction, using ordinary oligonucleotides without labeling or modification.

摘要

杂交链式反应（HCR）为无需目标扩增的核酸检测提供了一种可行的解决方案。通过高度特异性的夹心杂交，目标RNA可直接捕获到固体支持物上，并使用带有荧光染料的HCR进行检测。在此，我们描述了一种基于夹心杂交和二维HCR的新型疟疾RNA检测方法，该方法无需核酸纯化或任何酶促反应，使用未标记或修饰的普通寡核苷酸。