van der Veen R C, Trotter J L, Clark H B, Kapp J A
Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110.
J Neuroimmunol. 1989 Feb;21(2-3):183-91. doi: 10.1016/0165-5728(89)90174-4.
In this report we describe the transfer of chronic relapsing experimental allergic encephalomyelitis (EAE) with in vitro-stimulated lymph node cells (LNC) from SJL/J mice immunized with human myelin proteolipid protein (PLP). No additional immune enhancing procedures were applied in the transfer recipients. Clinical and histological EAE was transferred with 10-30 X 10(6) LNC to 27/28 mice. The LNC proliferated in vitro to PLP, but not to myelin basic protein (MBP), and induced delayed-type hypersensitivity. Enrichment for lymphoblasts by Ficoll centrifugation was essential for the disease development. The clinical course usually showed an early episode of acute paralytic illness, followed by chronic relapsing disease, and resembled the transfer of EAE using MBP-specific cells, both clinically and histologically.
在本报告中，我们描述了用体外刺激的来自用人髓磷脂蛋白脂蛋白（PLP）免疫的SJL/J小鼠的淋巴结细胞（LNC）转移慢性复发性实验性自身免疫性脑脊髓炎（EAE）。在转移受体中未应用额外的免疫增强程序。用10 - 30×10⁶个LNC将临床和组织学EAE转移至27/28只小鼠。这些LNC在体外对PLP增殖，但对髓磷脂碱性蛋白（MBP）不增殖，并诱导迟发型超敏反应。通过Ficoll离心富集成淋巴细胞对疾病发展至关重要。临床病程通常表现为早期急性麻痹性疾病发作，随后是慢性复发性疾病，在临床和组织学上都类似于使用MBP特异性细胞转移EAE。
