一种用于检测尿液中人绒毛膜促性腺激素的改良乳胶凝集抑制试验。
An improved latex agglutination-inhibition test for the detection of human chorionic gonadotropin in urine.
作者信息
Lim P L, Choy W F
机构信息
Department of Microbiology, University of Hong Kong.
出版信息
J Immunol Methods. 1989 Feb 8;117(1):137-9. doi: 10.1016/0022-1759(89)90128-2.
A commercially available slide latex agglutination-inhibition test for pregnancy was used in a tube modification for the detection of human chorionic gonadotropin (hCG) in urine. The tube method used 100 microliters sample and small quantities of reagents which were mixed continuously for 30 min. The results obtained, based on flocculation, were 15-30-fold better than those obtained by slide agglutination using spiked samples or urine samples from pregnant women. The lowest concentration of hCG detected in the spiked samples was 31 IU/l. When 43 clinical specimens were examined, the tube method detected four more samples than the 18 detected by the slide method. A good correlation (r = 0.96) was observed between the two methods in the determination of the hCG content in the positive samples.
一种市售的妊娠玻片乳胶凝集抑制试验经改良后用于试管法检测尿中的人绒毛膜促性腺激素（hCG）。试管法使用100微升样本和少量试剂，将其持续混合30分钟。基于絮凝得到的结果比使用加标样本或孕妇尿液样本通过玻片凝集法得到的结果好15至30倍。加标样本中检测到的hCG最低浓度为31 IU/l。在检查43份临床标本时，试管法比玻片法多检测出4份样本。在测定阳性样本中的hCG含量时，两种方法之间观察到良好的相关性（r = 0.96）。
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