König Anke, Zöller Nadja, Kippenberger Stefan, Bernd August, Kaufmann Roland, Layer Paul G, Heselich Anja
Goethe-University Medical School, Department of Dermatology, Venereology and Allergology, Frankfurt/Main, Germany.
Goethe-University Medical School, Department of Dermatology, Venereology and Allergology, Frankfurt/Main, Germany.
J Photochem Photobiol B. 2018 Jan;178:115-123. doi: 10.1016/j.jphotobiol.2017.11.003. Epub 2017 Nov 10.
Ionizing and near-infrared radiation are both part of the therapeutic spectrum in cancer treatment. During cancer therapy ionizing radiation is typically used for non-invasive reduction of malignant tissue, while near-infrared photobiomodulation is utilized in palliative medical approaches, e.g. for pain reduction or impairment of wound healing. Furthermore, near-infrared is part of the solar wavelength spectrum. A combined exposure of these two irradiation qualities - either intentionally during medical treatment or unintentionally due to solar exposure - is therefore presumable for cancer patients. Several studies in different model organisms and cell cultures show a strong impact of near-infrared pretreatment on ionizing radiation-induced stress response. To investigate the risks of non-thermal near-infrared (NIR) pretreatment in patients, a human in vitro full thickness skin models (FTSM) was evaluated for radiation research. FTSM were pretreated with therapy-relevant doses of NIR followed by X-radiation, and then examined for DNA-double-strand break (DSB) repair, cell proliferation and apoptosis. Double-treated FTSM revealed a clear influence of NIR on X-radiation-induced stress responses in cells in their typical tissue environment. Furthermore, over a 24h time period, double-treated FTSM presented a significant persistence of DSBs, as compared to samples exclusively irradiated by X-rays. In addition, NIR pretreatment inhibited apoptosis induction of integrated fibroblasts, and counteracted the radiation-induced proliferation inhibition of basal keratinocytes. Our work suggests that cancer patients treated with X-rays should be prevented from uncontrolled NIR irradiation. On the other hand, controlled double-treatment could provide an alternative therapy approach, exposing the patient to less radiation.
电离辐射和近红外辐射都是癌症治疗中治疗光谱的一部分。在癌症治疗期间，电离辐射通常用于非侵入性地减少恶性组织，而近红外光生物调节则用于姑息性医疗方法，例如减轻疼痛或改善伤口愈合。此外，近红外是太阳光谱的一部分。因此，癌症患者可能会有意在医疗过程中或因太阳照射而意外地同时暴露于这两种辐射特性下。在不同模式生物和细胞培养中的多项研究表明，近红外预处理对电离辐射诱导的应激反应有强烈影响。为了研究患者中非热近红外（NIR）预处理的风险，评估了一种用于辐射研究的人体体外全层皮肤模型（FTSM）。FTSM先用与治疗相关剂量的NIR预处理，然后进行X射线照射，然后检测DNA双链断裂（DSB）修复、细胞增殖和凋亡情况。经过双重处理的FTSM显示，在其典型组织环境中，NIR对细胞中X射线诱导的应激反应有明显影响。此外，在24小时的时间段内，与仅接受X射线照射的样本相比，经过双重处理的FTSM中DSB有显著的持续性。此外，NIR预处理抑制了整合成纤维细胞的凋亡诱导，并抵消了辐射诱导的基底角质形成细胞的增殖抑制。我们的研究表明，接受X射线治疗的癌症患者应避免不受控制的近红外辐射。另一方面，可控的双重治疗可以提供一种替代治疗方法，使患者接受更少的辐射。
