Physical Chemistry, Department of Chemistry, Lund University, P.O. Box 124, SE-22100, Lund, Sweden.
Camurus AB, Ideon Science Park, Gamma Building, Sölvegatan 41, SE-22379, Lund, Sweden(1); Biomedical Science, Faculty of Health and Society, Malmö University, SE-20506, Malmö, Sweden.
Chem Phys Lipids. 2018 Mar;211:86-92. doi: 10.1016/j.chemphyslip.2017.11.011. Epub 2017 Nov 11.
The effects of different lipolytic enzymes on the structure of lipid liquid crystalline nano-particles (LCNP) have been investigated by cryogenic transmission electron microscopy (cryo-TEM) and synchrotron small angle X-ray diffraction (SAXD). Here we used highly structured cubic micellar (Fd3m) nanoparticles of 50/50 (wt%/wt%) soy phosphatidyl choline (SPC)/glycerol dioleate (GDO) as substrate. Two types of lipolytic enzymes were used, phospholipase A (PLA) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO. Evolution of the structure was found to be very different and linked to specificity of the two types of enzymes. PLA, which hydrolyses the lamellar forming component, SPC, induces a reversed micellar lipid phase, while TGL which hydrolysis the reverse phase forming compound, GDO, induces a lamellar phase.
通过低温透射电子显微镜（cryo-TEM）和同步加速器小角 X 射线衍射（SAXD）研究了不同脂肪酶对脂质液晶纳米粒子（LCNP）结构的影响。 在这里，我们使用高度结构化的立方胶束（Fd3m）50/50（wt％/ wt％）大豆磷脂酰胆碱（SPC）/甘油二油酸酯（GDO）纳米颗粒作为底物。 使用了两种类型的脂肪酶，即催化磷脂成分 SPC 降解的磷脂酶 A（PLA）和促进甘油二酯水解的猪胰腺三酰基甘油脂肪酶（TGL）。 发现结构的演变非常不同，并且与两种类型的酶的特异性有关。 水解层状形成成分 SPC 的 PLA 诱导反向胶束脂质相，而水解反向相形成化合物 GDO 的 TGL 则诱导层状相。
