Ribeiro Naiara C S, Campos Mônica S, Santos Mariana B, Ayusso Gabriela M, Vilamaior Patrícia S L, Regasini Luis O, Taboga Sebastião R, Biancardi Manoel F, Perez Ana P S, Santos Fernanda C A
Department of Histology, Embryology and Cell Biology, Laboratory of Histophysiology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás 74001970, Brazil.
Department of Biology, Laboratory of Microscopy and Microanalysis, State University of São Paulo - UNESP, Rua Cristóvão Colombo, 2265, São José do Rio Preto, São Paulo 15054000, Brazil.
Fitoterapia. 2018 Jan;124:137-144. doi: 10.1016/j.fitote.2017.11.003. Epub 2017 Nov 10.
Chrysin is a plant-derived polyphenol that has the potential to increase endogenous testosterone levels both by inhibiting the aromatase enzyme and by stimulating testicular steroidogenesis. The effects of chrysin on the prostate are unknown, especially during its development and functional maturation. Thus, the aim of this study was to evaluate the effects of chrysin prepubertal exposure on the male and female prostates of both pubertal and adult gerbils. To evaluate the possible androgenic responses of chrysin, gerbils were also exposed to testosterone. Male and female gerbils were exposed to chrysin or to testosterone cypionate from postnatal day 15 to 42. Male and female gerbils were euthanized at either 43days or 90days age. The prostates were collected for biometrical, morphological and immunohistochemical analysis. The results showed that prepubertal exposure to chrysin had differential effects on the prostate of both pubertal and adult animals. The prostates of male and female pubertal gerbils showed no histological alterations, although there was increased frequency of androgen receptor (AR) in males and females, and estrogen receptor alpha (ERα) in females. Adult males and females presented developed prostate glands, with higher cell proliferative rate. In addition, AR and ERα frequency remained high in the prostate of adult animals. These results demonstrated that prepubertal exposure to chrysin disrupts steroid receptors regulation in the prostate, potentiating the response of this gland to the biological effects of endogenous steroids. In this context, excessive consumption of phytoestrogens during the critical stages of development should be considered with caution.
白杨素是一种植物来源的多酚，它有可能通过抑制芳香化酶和刺激睾丸类固醇生成来提高内源性睾酮水平。白杨素对前列腺的影响尚不清楚，尤其是在其发育和功能成熟过程中。因此，本研究的目的是评估青春期前暴露于白杨素对青春期和成年沙鼠雄性和雌性前列腺的影响。为了评估白杨素可能的雄激素反应，沙鼠也被暴露于睾酮。雄性和雌性沙鼠从出生后第15天到42天暴露于白杨素或环丙孕酮睾酮。雄性和雌性沙鼠在43天或90天龄时安乐死。收集前列腺进行生物测量、形态学和免疫组织化学分析。结果表明，青春期前暴露于白杨素对青春期和成年动物的前列腺有不同影响。雄性和雌性青春期沙鼠的前列腺没有组织学改变，尽管雄性和雌性的雄激素受体（AR）频率增加，雌性的雌激素受体α（ERα）频率增加。成年雄性和雌性的前列腺发达，细胞增殖率更高。此外，成年动物前列腺中AR和ERα的频率仍然很高。这些结果表明，青春期前暴露于白杨素会破坏前列腺中类固醇受体的调节，增强该腺体对内源性类固醇生物效应的反应。在这种情况下，在发育的关键阶段应谨慎考虑过量食用植物雌激素。
