Geisler Christoph, Jarvis Donald L
GlycoBac LLC, 1938 Harney Street, Laramie, WY 82072, USA.
GlycoBac LLC, 1938 Harney Street, Laramie, WY 82072, USA; University of Wyoming, Department of Molecular Biology, 1000 E. University Avenue, Laramie, WY 82071, USA.
Protein Expr Purif. 2018 Apr;144:25-32. doi: 10.1016/j.pep.2017.11.002. Epub 2017 Nov 10.
Insect cells are widely used for recombinant protein expression, typically as hosts for recombinant baculovirus vectors, but also for plasmid-mediated transient transfection or stable genetic transformation. Insect cells are used to express proteins for research, as well as to manufacture biologicals for human and veterinary medicine. Recently, several insect cell lines used for recombinant protein expression were found to be persistently infected with adventitious viruses. This has raised questions about how these infections might affect research performed using those cell lines. Furthermore, these findings raised serious concerns about the safety of biologicals produced using those cell lines. In response, new insect cell lines lacking adventitious viruses have been isolated for use as improved research tools and safer biological manufacturing platforms. Here, we review the scientific and patent literature on adventitious viruses found in insect cell lines, affected cell lines, and new virus-free cell lines.
昆虫细胞被广泛用于重组蛋白表达，通常作为重组杆状病毒载体的宿主，也用于质粒介导的瞬时转染或稳定遗传转化。昆虫细胞用于表达研究用蛋白，以及生产用于人类和兽医学的生物制品。最近，发现几种用于重组蛋白表达的昆虫细胞系持续感染了外源病毒。这引发了关于这些感染如何影响使用这些细胞系进行的研究的问题。此外，这些发现引发了对使用这些细胞系生产的生物制品安全性的严重担忧。作为回应，已分离出缺乏外源病毒的新型昆虫细胞系，用作改进的研究工具和更安全的生物制造平台。在这里，我们综述了关于在昆虫细胞系、受影响的细胞系和新型无病毒细胞系中发现的外源病毒的科学和专利文献。
