Wu Yan, Xue Chunyan, Lu Yan, Huang Zhenping
Department of Ophthalmology, Jinling Medical School of Nanjing Medical University; Department of Ophthalmology, Jinling Hospital, Nanjing 210002, Jiangsu Province, PR China.
Department of Ophthalmology, Jinling Hospital, Nanjing 210002, Jiangsu Province, PR China.
Indian J Ophthalmol. 2017 Nov;65(11):1127-1132. doi: 10.4103/ijo.IJO_339_17.
The aim of this study was to evaluate the inhibitory effect of different concentrations of KH902 eye drops on rabbit corneal neovascularization (CNV) induced by alkali burn.
Forty-eight adult rabbits were randomized into four groups after alkali burning: Group A (2.5 mg/ml), Group B (5 mg/ml), and Group C (10 mg/ml) by different concentrations of KH902 eye drops and Group D by saline solution as control with three times a day for 2 weeks. At days 7, 14, and 28, the anterior segment photographs, confocal microscopy, and histopathology were performed to evaluate corneal opacity, neovascularization, inflammatory cell density, vessel size, and edema. Immunohistochemistry was applied to analyze the vascular endothelial growth factor (VEGF) level.
(1) The CNV in the medicine-treated groups showed a reduction without obvious corneal side effects histologically. (2) Compared to the control group, the three medicine-treated groups showed a reduction in the VEGF levels and CNV areas on days 7, 14, and 28 and in the inflammatory cell density on days 14 and 28 (P < 0.01). The difference of inflammatory cell density between the three medicine-treated groups existed on day 14 (P < 0.01). There were differences in the VEGF levels between Groups A, B, and C on days 7, 14, and 28 (P < 0.01), not for Groups B and C on day 28 (P > 0.05).
KH902 eye drops in lower concentration showed an obvious reduction of the CNV growing for rabbit corneal alkali burn without side effects.
本研究旨在评估不同浓度的KH902滴眼液对碱烧伤诱导的兔角膜新生血管化（CNV）的抑制作用。
48只成年兔碱烧伤后随机分为四组：A组（2.5mg/ml）、B组（5mg/ml）和C组（10mg/ml），分别给予不同浓度的KH902滴眼液，D组给予生理盐水作为对照，每天滴眼3次，共2周。在第7、14和28天，进行眼前节照相、共聚焦显微镜检查和组织病理学检查，以评估角膜混浊、新生血管化、炎症细胞密度、血管大小和水肿情况。应用免疫组织化学分析血管内皮生长因子（VEGF）水平。
（1）药物治疗组的CNV在组织学上显示减少，且无明显角膜副作用。（2）与对照组相比，三个药物治疗组在第7、14和28天的VEGF水平和CNV面积以及在第14和28天的炎症细胞密度均降低（P<0.01）。三个药物治疗组在第14天的炎症细胞密度存在差异（P<0.01）。A、B、C组在第7、14和28天的VEGF水平存在差异（P<0.01），B组和C组在第28天无差异（P>0.05）。
低浓度的KH902滴眼液可明显减少兔角膜碱烧伤后CNV的生长，且无副作用。
