Shu Jiaze, Li Defu, Ouyang Haiping, Huang Junyi, Long Zhen, Liang Zhihao, Chen Yuqin, Chen Yiguan, Zheng Qiuyu, Kuang Meidan, Tang Haiyang, Wang Jian, Lu Wenju
State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, Guangzhou, Guangdong, 510120, P.R. China.
Division of Translational and Regenerative Medicine, Department of Medicine and Department of Physiology, The University of Arizona College of Medicine, Tucson, Arizona, United States.
Sci Rep. 2017 Nov 13;7(1):15454. doi: 10.1038/s41598-017-15685-y.
Animal model of cigarette smoke (CS) -induced chronic obstructive pulmonary disease (COPD) is the primary testing methodology for drug therapies and studies on pathogenic mechanisms of disease. However, researchers have rarely run simultaneous or side-by-side tests of whole-body and nose-only CS exposure in building their mouse models of COPD. We compared and evaluated these two different methods of CS exposure, plus airway Lipopolysaccharides (LPS) inhalation, in building our COPD mouse model. Compared with the control group, CS exposed mice showed significant increased inspiratory resistance, functional residual capacity, right ventricular hypertrophy index, and total cell count in BALF. Moreover, histological staining exhibited goblet cell hyperplasia, lung inflammation, thickening of smooth muscle layer on bronchia, and lung angiogenesis in both methods of CS exposure. Our data indicated that a viable mouse model of COPD can be established by combining the results from whole-body CS exposure, nose-only CS exposure, and airway LPS inhalation testing. However, in our study, we also found that, given the same amount of particulate intake, changes in right ventricular pressure and intimal thickening of pulmonary small artery are a little more serious in nose-only CS exposure method than changes in the whole-body CS exposure method.
香烟烟雾（CS）诱导的慢性阻塞性肺疾病（COPD）动物模型是药物治疗和疾病致病机制研究的主要测试方法。然而，研究人员在构建COPD小鼠模型时，很少同时或并行进行全身和仅鼻腔CS暴露试验。我们在构建COPD小鼠模型时，比较并评估了这两种不同的CS暴露方法，以及气道内吸入脂多糖（LPS）的方法。与对照组相比，CS暴露小鼠的吸气阻力、功能残气量、右心室肥大指数和支气管肺泡灌洗液（BALF）中的细胞总数显著增加。此外，组织学染色显示，两种CS暴露方法均出现杯状细胞增生、肺部炎症、支气管平滑肌层增厚和肺部血管生成。我们的数据表明，结合全身CS暴露、仅鼻腔CS暴露和气道LPS吸入试验的结果，可以建立可行的COPD小鼠模型。然而，在我们的研究中，我们还发现，在吸入相同量颗粒物的情况下，仅鼻腔CS暴露方法引起的右心室压力变化和肺小动脉内膜增厚比全身CS暴露方法更严重一些。
