College of Environment, Zhejiang University of Technology, Hangzhou, 310014, China.
Institute of Quality and Standard for Agro-products, State Key Laboratory Breeding Base Zhejiang Sustainable, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, Zhejiang, People's Republic of China.
Environ Sci Pollut Res Int. 2018 Jan;25(3):2718-2725. doi: 10.1007/s11356-017-0539-4. Epub 2017 Nov 13.
The enantioselective degradation of myclobutanil and famoxadone enantiomers in grape under open field was investigated in this study. The absolute configuration of myclobutanil and famoxadone enantiomers was determined by the combination of experimental electronic circular dichroism (ECD) and calculated ECD spectra. The enantiomers residues of myclobutanil and famoxadone in grape were measured by sensitive high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS). The linearity, precision, accuracy, matrix effect, and stability were assessed. And the limit of quantification (LOQ) for each enantiomer of myclobutanil and famoxadone in grape was evaluated to be 1.5 and 2 μg kg. The myclobutanil and famoxadone showed the enantioselective degradation in grape, and the enantioselectivity of degradation for myclobutanil was more pronounced than that for famoxadone. The half-lives were 13.1 days and 25.7 days for S-(+)-myclobutanil and R-(-)-myclobutanil in grape, separately. The half-life of S-(+)-famoxadone was 31.5 days slightly shorter than that of R-(-)-famoxadone with half-life being 38.5 days in grape. The probable reasons for the enantioselective degradation behavior of these two fungicides were also discussed. The results in the article might provide a reference to better assess the risks of myclobutanil and famoxadone enantiomers in grapes to human and environment. Graphical abstract The enantioselective analysis of myclobutanil and famoxadone in grape.
本研究考察了咪鲜胺和唑螨酯对田间葡萄中对映体的选择性降解。通过实验电子圆二色性（ECD）和计算ECD 光谱的结合，确定了咪鲜胺和唑螨酯对映体的绝对构型。采用灵敏的高效液相色谱/串联质谱（HPLC-MS/MS）测定了葡萄中咪鲜胺和唑螨酯对映体的残留量。评估了线性、精密度、准确度、基质效应和稳定性。并评价了咪鲜胺和唑螨酯在葡萄中每个对映体的定量限（LOQ）分别为 1.5 和 2μg/kg。咪鲜胺和唑螨酯在葡萄中表现出对映体选择性降解，咪鲜胺的对映体选择性降解比唑螨酯更明显。咪鲜胺 S-(+)-对映体和 R-(-)-对映体在葡萄中的半衰期分别为 13.1 天和 25.7 天。S-(+)-唑螨酯的半衰期为 31.5 天，略短于 R-(-)-唑螨酯的半衰期 38.5 天。还讨论了这两种杀菌剂对映体选择性降解行为的可能原因。本文的结果可能为更好地评估咪鲜胺和唑螨酯对映体在葡萄中对人类和环境的风险提供参考。
