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噬菌体特性描述的基本步骤:生物学、分类学和基因组分析

Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis.

作者信息

Aziz Ramy Karam, Ackermann Hans-Wolfgang, Petty Nicola K, Kropinski Andrew M

机构信息

Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Qasr El-Ainy, 11562, Cairo, Egypt.

Department of Microbiology, Immunology, and Infectiology, Faculty of Medicine, Université Laval, Quebec, QC, Canada, G1X 4C6.

出版信息

Methods Mol Biol. 2018;1681:197-215. doi: 10.1007/978-1-4939-7343-9_15.

Abstract

Because of the rise in antimicrobial resistance there has been a significant increase in interest in phages for therapeutic use. Furthermore, the cost of sequencing phage genomes has decreased to the point where it is being used as a teaching tool for genomics. Unfortunately, the quality of the descriptions of the phage and its annotation frequently are substandard. The following chapter is designed to help people working on phages, particularly those new to the field, to accurately describe their newly isolated viruses.

摘要

由于抗菌素耐药性的增加,噬菌体在治疗用途方面的关注度显著提高。此外,噬菌体基因组测序的成本已降至可作为基因组学教学工具的程度。不幸的是,噬菌体描述及其注释的质量常常不达标。以下章节旨在帮助从事噬菌体研究的人员,尤其是该领域的新手,准确描述他们新分离出的病毒。

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