Pope Welkin H, Jacobs-Sera Deborah
Department of Biological Sciences, University of Pittsburgh, 367 Crawford Hall, 4249, 5th Ave., Pittsburgh, PA, 15260, USA.
Methods Mol Biol. 2018;1681:217-229. doi: 10.1007/978-1-4939-7343-9_16.
Current sequencing technologies allow for the rapid and inexpensive sequencing of complete bacteriophage genomes, using small quantities of nucleic acid as starting material. Determination of the location and function of the gene features within the genome sequence, or annotation, is a necessary next step prior to submission to a public database, publication in a scientific journal, or advanced comparative genomic and proteomic studies. Gene prediction can be largely accomplished through the use of several freely available programs. However, manual inspection and refinement is essential to the production of the most accurate genome annotations. Here, we describe an overview of the annotation of a bacteriophage genome sequence using the freely available program DNA Master.
当前的测序技术能够以少量核酸作为起始材料，快速且低成本地对完整的噬菌体基因组进行测序。在将基因组序列提交到公共数据库、发表于科学期刊或开展高级比较基因组学和蛋白质组学研究之前，确定基因组序列中基因特征的位置和功能，即注释，是必要的下一步。基因预测在很大程度上可以通过使用几个免费可用的程序来完成。然而，人工检查和完善对于生成最准确的基因组注释至关重要。在这里，我们概述了使用免费程序DNA Master对噬菌体基因组序列进行注释的过程。
