Haapa-Paananen Saija, Savilahti Harri
Division of Genetics and Physiology, Department of Biology, University of Turku, Turku, FI-20014, Finland.
Methods Mol Biol. 2018;1681:279-286. doi: 10.1007/978-1-4939-7343-9_20.
The capacity of transposable elements to insert into the genomes has been harnessed during the past decades to various in vitro and in vivo applications. This chapter describes in detail the general protocols and principles applicable for the Mu in vitro transposition reaction as well as the assembly of DNA transposition complexes that can be electroporated into bacterial cells to accomplish efficient gene delivery. These techniques with their modifications potentiate various gene and genome modification applications, which are discussed briefly here, and the reader is referred to the original publications for further details.
在过去几十年中，转座元件插入基因组的能力已被应用于各种体外和体内实验。本章详细描述了适用于Mu体外转座反应的一般方案和原则，以及可通过电穿孔导入细菌细胞以实现高效基因传递的DNA转座复合物的组装。这些技术及其改进方法增强了各种基因和基因组修饰应用，本文对此进行了简要讨论，读者可参考原始出版物以获取更多详细信息。
