Frontier Center for Organic Materials, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata, 992-8510, Japan.
Innovative Flex Course for Frontier Organic Materials Systems, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata, 992-8510, Japan.
Macromol Biosci. 2017 Dec;17(12). doi: 10.1002/mabi.201700297. Epub 2017 Nov 14.
Chondrocytes are important for cartilage tissue engineering. However, dedifferentiation during chondrocyte subculture prevents the application of cartilage tissue engineering. Therefore, prevention of this dedifferentiation is required. Here, the possibility of poly(2-methoxyethyl acrylate) (PMEA) and its analogous polymers, poly(tetrahydrofurfuryl acrylate) (PTHFA) and poly(2-(2-methoxyethoxy) ethyl acrylate-co-butyl acrylate) (PMe2A), for chondrocyte subculture without dedifferentiation is examined. Chondrocytes spread on PTHFA and polyethylene terephthalate (PET), whereas their spreading is delayed on PMEA and PMe2A. When primary chondrocytes are subcultured on these polymers, the expression levels of cartilaginous genes are higher on PMEA and PMe2A than on PET and PTHFA. Integrin contribution to the initial cell adhesion is lower on PMEA and PMe2A than on PTHFA and PET. This low level of integrin contribution to cell adhesion may cause a delay in cell spreading and the maintenance of cartilaginous gene expression. These results indicate that PMEA and PMe2A may be favorable substrates for chondrocyte subculture and cartilage tissue engineering.
软骨细胞对于软骨组织工程非常重要。然而，软骨细胞在传代培养过程中的去分化会阻止软骨组织工程的应用。因此，需要防止这种去分化。在这里，研究了聚（2-甲氧基乙酯基丙烯酸酯）（PMEA）及其类似物聚合物聚（四氢呋喃基丙烯酸酯）（PTHFA）和聚（2-（2-甲氧基乙氧基）乙基丙烯酸酯-共-丙烯酸丁酯）（PMe2A）在软骨细胞传代培养中不发生去分化的可能性。软骨细胞在 PTHFA 和聚对苯二甲酸乙二醇酯（PET）上扩散，而在 PMEA 和 PMe2A 上扩散则延迟。当原代软骨细胞在这些聚合物上进行传代培养时，PMEA 和 PMe2A 上软骨基因的表达水平高于 PET 和 PTHFA。整合素对初始细胞黏附的贡献在 PMEA 和 PMe2A 上低于 PTHFA 和 PET。这种低水平的整合素对细胞黏附的贡献可能导致细胞扩散延迟和软骨基因表达的维持。这些结果表明，PMEA 和 PMe2A 可能是软骨细胞传代培养和软骨组织工程的有利底物。
