Tanko Y, Salisu A I, Mohammed K A, Musa S A, Jimoh A, Yusuf R
Department of Human Physiology, Ahmadu Bello University, Zaria.
Niger J Physiol Sci. 2017 Jun 30;32(1):91-96.
The present study investigated the anti-hyperglycaemic effect of rutin on blood glucose, oxidative stressbiomarkers and lipid peroxidation in alloxan induced hyperglycaemic wistar rats. Diabetes was induced in rats by anintraperitoneal (i.p) injection of alloxan monohydrate 150 mg/kg body weight. Twenty five wistar rats were divided asfollows; Group1 served as diabetic control received distilled water 2 mg/kg, Group served as positive control received 2mg/kg glibenclamide, 3, 4 and 5 received rutin at 50, 100 and 200 mg/kg body weight for 28 days respectively. At the end of the treatment, rats were sacrificed and the blood and serum were used for the analysis of blood glucose and oxidativestress biomarkers respectively. The determinations of blood glucose levels were carried out at intervals of 7, 14, 21 and 28days respectively Serum oxidative stress biomarkers lipid peroxidation, were done on the 28 days. Administrations of rutinat the three different doses 50,100 and 200 mg/kg to diabetic rats significantly (p<0.05) decreased the blood glucose levelsas compared to diabetic control. The dose of 200 mg/kg exhibited a maximum glucose lowering effect with blood glucoseof 102.8± 0.06 as compared to diabetic control 346.2±0.16. Furthermore, in relation to the oxidative stress biomarkers therewas a significant (p<0.05) increased in the levels of gluthathione peroxidase, superoxide dismutase and catalase as comparedto control. However, there was also a significant decreased in the malondialdehyde levels as compared to control. It may beconcluded that oral administration of Rutin for 28 days decreases blood glucose levels and prevented oxidative stress andantioxidant status in hyperglycaemic rats.
本研究调查了芦丁对四氧嘧啶诱导的高血糖Wistar大鼠的血糖、氧化应激生物标志物和脂质过氧化的抗高血糖作用。通过腹腔注射150mg/kg体重的一水合四氧嘧啶在大鼠中诱导糖尿病。将25只Wistar大鼠分为以下几组：第1组作为糖尿病对照组，接受2mg/kg的蒸馏水；第2组作为阳性对照组，接受2mg/kg的格列本脲；第3、4和5组分别接受50、100和200mg/kg体重的芦丁，持续28天。治疗结束时，处死大鼠，血液和血清分别用于分析血糖和氧化应激生物标志物。血糖水平的测定分别在第7、14、21和28天进行。血清氧化应激生物标志物脂质过氧化在第28天进行测定。给糖尿病大鼠分别给予50、100和200mg/kg这三种不同剂量的芦丁后，与糖尿病对照组相比，血糖水平显著降低（p<0.05）。200mg/kg剂量表现出最大的降糖效果，血糖为102.8±0.06，而糖尿病对照组为346.2±0.16。此外，与对照组相比，氧化应激生物标志物谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶的水平显著升高（p<0.05）。然而，与对照组相比，丙二醛水平也显著降低。可以得出结论，口服芦丁28天可降低高血糖大鼠的血糖水平，并预防氧化应激和抗氧化状态。
