Postgraduate Program in Human Nutrition, Faculty of Health Sciences, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília 70910-900, Brazil.
Department of Nutrition, Faculty of Health Sciences, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília 70910-900, Brazil.
Nutrients. 2017 Nov 14;9(11):1243. doi: 10.3390/nu9111243.
Aging may be related to oxidative damage accumulation and a low-grade inflammation, both responses are modulated by iron and phytochemicals. This study investigated the effect of tucum-do-cerrado ( Mart.) consumption on the expression of sirtuins (SIRT 1 and 3) and senescence marker protein-30 (SMP30), and on the redox and inflammatory responses, in adult rats supplemented or not with dietary iron. Male Wistar rats were treated for 12 weeks with: control diet (CT); iron enriched-diet (+Fe); control diet + 15% tucum-do-cerrado (Tuc); or iron enriched-diet + 15% tucum-do-cerrado (Tuc + Fe). Iron supplementation (+Fe) increased liver, spleen and intestine iron levels, transferrin saturation, serum iron, serum TNF-α and IL-6 levels, hepatic carbonyl content and and superoxide dismutase (SOD) activity, hepatic Nrf2 protein and Nqo1 mRNA levels and decreased the renal Sirt1 mRNA levels in relation to CT group. Tucum-do-cerrado consumption (Tuc) increased hepatic SOD activity, Nrf2 and SIRT1 mRNA and protein contents, and Nqo1 mRNA levels, while it decreased the renal SOD activity compared with the CT diet. The consumption of tucum-do-cerrado associated with the iron-enriched diet (Tuc + Fe) increased the iron levels in tissues and serum transferrin saturation, compared to the CT diet, while promoting a decrease in hepatic carbonyl and renal malondialdehyde levels, marginally reducing serum IL-6 levels, and increasing hepatic SIRT1 protein content, renal Sirt1 and hepatic Nrf2 mRNA levels, compared to the +Fe group. None of the treatments altered Smp30 mRNA levels. The results suggest that tucum-do-cerrado consumption might promote an anti-aging effect by increasing SIRT1 expression, which may enhance Nrf2 mRNA and protein levels and its downstream pathway, which in turn decrease oxidative damage to proteins and the levels of inflammatory cytokines (IL-6 and TNF-α), induced by iron excess.
衰老是与氧化损伤积累和低度炎症有关，这两种反应都受铁和植物化学物质的调节。本研究调查了食用塔库姆-多-塞拉多（Mart.）对成年大鼠（补充或不补充膳食铁）中沉默调节蛋白（SIRT1 和 SIRT3）和衰老标志物蛋白-30（SMP30）的表达、氧化还原和炎症反应的影响。雄性 Wistar 大鼠用以下方法处理 12 周：对照饮食（CT）；富含铁的饮食（+Fe）；对照饮食+15%塔库姆-多-塞拉多（Tuc）；或富含铁的饮食+15%塔库姆-多-塞拉多（Tuc+Fe）。铁补充剂（+Fe）增加了肝脏、脾脏和肠道中的铁含量、转铁蛋白饱和度、血清铁、血清 TNF-α 和 IL-6 水平、肝羰基含量和超氧化物歧化酶（SOD）活性、肝 Nrf2 蛋白和 Nqo1 mRNA 水平，并降低了与 CT 组相比，肾 Sirt1 mRNA 水平。塔库姆-多-塞拉多的消耗（Tuc）增加了肝 SOD 活性、Nrf2 和 SIRT1 mRNA 和蛋白含量以及 Nqo1 mRNA 水平，而与 CT 饮食相比，肾 SOD 活性降低。与富含铁的饮食（Tuc+Fe）相比，塔库姆-多-塞拉多的消耗增加了组织和血清转铁蛋白饱和度中的铁含量，与 CT 饮食相比，还促进了肝羰基和肾丙二醛水平的降低，血清 IL-6 水平略有降低，肝 SIRT1 蛋白含量增加，肾 Sirt1 和肝 Nrf2 mRNA 水平增加，与+Fe 组相比。没有一种处理方法改变 Smp30 mRNA 水平。结果表明，塔库姆-多-塞拉多的消耗可能通过增加 SIRT1 的表达来促进抗衰老作用，这可能增强 Nrf2 mRNA 和蛋白水平及其下游途径，从而减少铁过量引起的蛋白质氧化损伤和炎症细胞因子（IL-6 和 TNF-α）的水平。
