Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, Sichuan, China (mainland).
Med Sci Monit. 2017 Nov 14;23:5420-5429. doi: 10.12659/msm.904681.
BACKGROUND The aim of this study was to explore the correlation between HLA-B27 and the intracellular elimination, replication, and trafficking of Salmonella enteritidis (S. enteritidis) collected from patients with reactive arthritis. MATERIAL AND METHODS Confocal microscopy, flow cytometry, and sandwich enzyme-linked immunosorbent assay (ELISA) were employed in this study to evaluate the localization of proteins of interest, to assess the intracellular trafficking of S. enteritidis, and to measure the production of cytokines of interest. RESULTS HLA-B27 was negatively associated with intracellular S. enteritidis elimination in healthy human monocytes/macrophages. In S. enteritidis infected monocytes/macrophages, HLA-27B was also negatively correlated with bacteria elimination but positively related to bacteria replication. S. enteritidis did not co-localize with NRAMP1 and LAMP1/2 in HLA-B27 cells. S. enteritidis did not co-exist with transferrin or dextran within HLA-B27 and A2 cells. CONCLUSIONS HLA-B27 is closely associated with the intracellular elimination and replication of S. enteritidis. Replicated bacteria in HLA-B27 monocytic cells were located within unique vacuoles rather than disturbing host endocytosis.
本研究旨在探讨从反应性关节炎患者中分离的沙门氏菌（S. enteritidis）的细胞内清除、复制和运输与 HLA-B27 之间的相关性。
本研究采用共聚焦显微镜、流式细胞术和夹心酶联免疫吸附试验（ELISA）来评估感兴趣蛋白的定位、沙门氏菌的细胞内运输以及感兴趣细胞因子的产生。
HLA-B27 与健康人单核细胞/巨噬细胞中的细胞内 S. enteritidis 清除呈负相关。在感染 S. enteritidis 的单核细胞/巨噬细胞中，HLA-27B 也与细菌清除呈负相关，但与细菌复制呈正相关。在 HLA-B27 细胞中，沙门氏菌与 NRAMP1 和 LAMP1/2 不共定位。在 HLA-B27 和 A2 细胞中，沙门氏菌与转铁蛋白或葡聚糖不共存。
HLA-B27 与 S. enteritidis 的细胞内清除和复制密切相关。在 HLA-B27 单核细胞内复制的细菌位于独特的空泡内，而不会干扰宿主内吞作用。
