Department of pollutant metrology, Institut National de Recherche et de Sécurité (INRS), 1 rue du Morvan, Vandœuvre-lès Nancy 54500, France.
Faculté des Sciences et Technologies, Institut National de la Recherche Agronomique, Dynamique des Génomes et Adaptation Microbienne (DynAMic), Université de Lorraine, UMR INRA 1128, Boulevard des Aiguillettes, BP 70239, F-54506 Vandoeuvre Cedex, France.
Ann Work Expo Health. 2017 Nov 10;61(9):1076-1086. doi: 10.1093/annweh/wxx075.
Waste sorting activities are source of occupational bioaerosol exposures that are associated with several health disorders. New analytical tools, based on next-generation sequencing (NGS) technologies, provide powerful methods to assess the microbial composition of bioaerosols. The objectives of the study were (i) to assess the feasibility and the repeatability of NGS-based biodiversity measurements and (ii) to study the microbial biodiversity using NGS in bioaerosols emitted in a waste sorting plant (WSP). Three stationary parallel samples were collected in a sorting cabin using closed-face cassettes equipped with polycarbonate membranes. Bacterial and fungal diversity was assessed by sequencing 16S and 18S rDNA genes using either Illumina sequencing or 454 pyrosequencing methods. At sampling point, airborne bacteria were dominated by Proteobacteria, Firmicutes, and Actinobacteria with prevailing genera assigned to unclassified Enterobacteriaceae, Staphylococcus, Acinetobacter, Leuconostoc, Pseudomonas, and Lactobacillus. Airborne fungi were dominated by Ascomycota with prevailing genera assigned to Penicillium, Aspergillus, Rhizopus, Wallemia, and Hemicarpenteles. The NGS biodiversity measurements revealed a higher biodiversity bioaerosols that previously reported for WSP in studies carried out using culture methods followed by identification of microorganisms. These results provide the first survey about taxonomic biodiversity in bioaerosols from WSPs using high-throughput sequencing.
垃圾分类活动是职业性生物气溶胶暴露的来源，与多种健康障碍有关。基于下一代测序 (NGS) 技术的新分析工具为评估生物气溶胶的微生物组成提供了强大的方法。本研究的目的是：(i) 评估基于 NGS 的生物多样性测量的可行性和可重复性；(ii) 使用 NGS 研究废物分拣厂 (WSP) 排放的生物气溶胶中的微生物生物多样性。使用装有聚碳酸酯膜的封闭面盒在分拣舱内采集了三个固定平行样本。通过使用 Illumina 测序或 454 焦磷酸测序方法对 16S 和 18S rDNA 基因进行测序，评估细菌和真菌多样性。在采样点，空气中的细菌主要由变形菌门、厚壁菌门和放线菌门组成，主要属归属于未分类肠杆菌科、葡萄球菌属、不动杆菌属、肠球菌属、假单胞菌属和乳杆菌属。空气中的真菌主要由子囊菌门组成，主要属归属于青霉属、曲霉属、根霉属、瓦勒姆氏菌属和半知菌属。NGS 生物多样性测量结果显示，生物气溶胶的生物多样性高于以前使用培养方法并随后对微生物进行鉴定的 WSP 研究报告的生物多样性。这些结果提供了使用高通量测序对 WSP 生物气溶胶进行分类生物多样性的首次调查。
