Chen Bin Bin, Sheng Li Rong, Li Liu Meng, Yan Zou Hong, Liu Hui, Huang Cheng Zhi
Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
Talanta. 2018 Feb 1;178:172-177. doi: 10.1016/j.talanta.2017.09.007. Epub 2017 Sep 5.
Determination of phosphate ion (PO) is important in biomedical and environmental arrays because its controlling concentrations are associated with different pathologies or the quality of water. Herein, we report a new type of photoluminescence (PL) probe for highly selective detection of PO based on a single-layered graphene quantum dots chelating with aluminium ions (s-GQDs-Al) system. The PL of s-GQDs can be enhanced by Al through the aggregation-induced emission enhancement (AIEE) effect. With the addition of PO, the PL of the s-GQDs-Al system is faded away because PO has stronger coordination with Al which results in the elimination of AIEE effect and the decrease in the PL intensity of the s-GQDs-Al system. Therefore, the s-GQDs-Al system can behave as an on-off type PL probe for PO detection. It is found that the PL intensity ratio (I/I) of s-GQDs in the presence of Al at 463nm is proportional to the concentration of PO in the range of 0.25-7.5μM with the limit of detection as low as 0.1μM. This selective assay has a great application prospect in the complex matrixes owing to its simplicity and specificity for PO detection.
磷酸根离子（PO）的测定在生物医学和环境领域中很重要，因为其浓度的控制与不同的病理状况或水质相关。在此，我们报道了一种基于单层石墨烯量子点与铝离子螯合（s-GQDs-Al）体系的新型光致发光（PL）探针，用于高选择性检测PO。Al可通过聚集诱导发光增强（AIEE）效应增强s-GQDs的PL。加入PO后，s-GQDs-Al体系的PL消失，因为PO与Al的配位更强，导致AIEE效应消除，s-GQDs-Al体系的PL强度降低。因此，s-GQDs-Al体系可作为一种用于PO检测的开-关型PL探针。研究发现，在463nm处存在Al时，s-GQDs的PL强度比（I/I）与0.25-7.5μM范围内的PO浓度成正比，检测限低至0.1μM。这种选择性检测方法因其对PO检测的简单性和特异性，在复杂基质中具有广阔的应用前景。
