Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Talanta. 2018 Feb 1;178:473-480. doi: 10.1016/j.talanta.2017.09.068. Epub 2017 Sep 28.
In this study, a novel generation of deep eutectic solvents (DESs) was used as an acceptor phase in three-phase hollow fiber liquid phase microextraction (HF-LPME) based on two immiscible organic phases. It was compared with other common DESs for extraction and preconcentration of dydrogesterone (DYD) and cyproterone acetate (CPA) from urine and plasma samples. The extracted analytes were analyzed by high performance liquid chromatography with UV-vis detector (HPLC-UV). This phosphonium based DES due to low volatility, low price and multifunctionality introduced itself as worthy next generation of acceptor phase in HF-LPME. The factors affected on extraction efficiency of the analytes were investigated and optimized. The performance of the proposed method was studied in terms of linear ranges (LRs from 1 to 500µgL with R ≥ 0.9946), precision (RSD% ≤ 6.3) and limits of detection (LODs in the range of 0.5-2µgL). Under the optimized conditions, preconcentration factors in the range of 187-428 were obtained. Finally, the method was applied to the analysis of DYD and CPA in human urine and plasma samples and desirable results were obtained.
在这项研究中，新型一代深共晶溶剂（DESs）被用作三相中空纤维液相微萃取（HF-LPME）的接受相，基于两相不混溶的有机相。与其他常见的 DESs 相比，该方法用于从尿液和血浆样品中萃取和预浓缩地屈孕酮（DYD）和醋酸环丙孕酮（CPA）。用带紫外可见检测器的高效液相色谱法（HPLC-UV）分析提取的分析物。由于具有挥发性低、价格低和多功能性，这种基于膦的 DES 有望成为 HF-LPME 中下一代有价值的接受相。研究并优化了影响分析物萃取效率的因素。该方法的性能在线性范围（LR 从 1 到 500µgL，R ≥ 0.9946）、精密度（RSD%≤6.3）和检测限（LOD 在 0.5-2µgL 范围内）方面进行了研究。在优化条件下，获得了 187-428 的浓缩因子。最后，该方法应用于人尿液和血浆样品中 DYD 和 CPA 的分析，得到了令人满意的结果。
