State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, China; College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022 China.
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, China; Centre of Analysis and Measurement, Jilin Institute of Chemical Technology, Jilin 132022 China.
Talanta. 2018 Feb 1;178:498-506. doi: 10.1016/j.talanta.2017.09.054. Epub 2017 Sep 20.
The technique of solid phase micro-extraction (SPME) is an important method for sample pretreatment in analytical chemistry, especially for the analysis in micro-systems. Surface enhanced Raman scattering (SERS) is an ultra-sensitive and fast detection technique. Both are particularly important in qualitative analysis of trace amount of substance. In this study, combining the magnetic nanoparticles with magnetic SPME device, we develop a high efficient new pretreatment method named as disperse magnetic solid phase micro-extraction (Dis-MSPME). In comparison to the traditional SPME, the proposed Dis-MSPME realizes solid phase micro-extraction from dispersive system, which improved the extraction efficiency largely. Conjunction the advantages both of Dis-MSPME and SERS is proposed as Dis-MSPME-SERS as a new detection method, which realize enrichment, magnetic separation and detection all-in-one. Making it a simpler, more efficient and sensitive approach in identifying the illegal additives. Sildenafil citrate (SC) in 500μL health wine as an example of illegal additive was successfully detected in a LOD of 1.0 × 10M. Moreover, comparative study on the extract efficient of Dis-MSPME-SERS with SPME-SERS shows it takes only 10min to detect sildenafil citrate in the health wine, from enrichment to detection by Dis-MSPME-SERS.
固相微萃取（SPME）技术是分析化学中样品预处理的重要方法，尤其适用于微系统分析。表面增强拉曼散射（SERS）是一种超灵敏和快速的检测技术。这两种技术在痕量物质的定性分析中都非常重要。在这项研究中，我们将磁性纳米粒子与磁性 SPME 装置结合，开发了一种高效的新预处理方法，称为分散固相微萃取（Dis-MSPME）。与传统的 SPME 相比，所提出的 Dis-MSPME 从分散体系中实现了固相微萃取，大大提高了萃取效率。结合 Dis-MSPME 和 SERS 的优点，提出了 Dis-MSPME-SERS 作为一种新的检测方法，它实现了富集、磁性分离和检测的一体化。这使得它成为一种更简单、更高效、更灵敏的识别非法添加剂的方法。以 500μL 保健酒中的西地那非（SC）为例，成功检测到非法添加剂的检出限为 1.0×10M。此外，Dis-MSPME-SERS 与 SPME-SERS 的萃取效率比较研究表明，Dis-MSPME-SERS 仅需 10min 即可从保健酒中检测到西地那非，从富集到检测。
