血清中 DNA 的高灵敏均相检测用单颗粒的散射测量
Scattering measurement of single particle for highly sensitive homogeneous detection of DNA in serum.
机构信息
Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, People's Republic of China; Department of Physics, Tsinghua University, Beijing 100084, People's Republic of China.
Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, People's Republic of China; Department of Physics, Tsinghua University, Beijing 100084, People's Republic of China.
出版信息
Talanta. 2018 Feb 1;178:545-551. doi: 10.1016/j.talanta.2017.09.052. Epub 2017 Sep 20.
A highly sensitive homogeneous method for DNA detection has been developed. The system relies on two kinds of gold nanorod (AuNR) probes with complementary DNA sequences to the target DNA. In the presence of the target DNA, two kinds of AuNR probes are assembling into dimers or small aggregates. The target-induced AuNR aggregate has higher scattering intensity than that of a single AuNR because of the plasmonic coupling effect. Dark field microscopy was utilized to image the single particle and measure its scattering intensity. We wrote our own Matlab code and used it to extract the scattering signal of all particles. Difference in distribution of scattering intensity between the single AuNR and its aggregate provides a quantitative basis for the detection of target DNA. A linear dynamic range spanning from 0.1pM to 1nM and a detection limit of ~ 30fM were achieved for the detection of DNA in serum sample.
已开发出一种高度灵敏的 DNA 检测均相方法。该系统依赖于两种与靶 DNA 互补的金纳米棒（AuNR）探针。在靶 DNA 的存在下，两种 AuNR 探针组装成二聚体或小聚集体。由于等离子体耦合效应，靶诱导的 AuNR 聚集体的散射强度比单个 AuNR 更高。暗场显微镜用于对单个颗粒成像并测量其散射强度。我们编写了自己的 Matlab 代码，并使用它来提取所有颗粒的散射信号。单个 AuNR 与其聚集体之间散射强度分布的差异为检测靶 DNA 提供了定量依据。在血清样本中检测 DNA 时，实现了从 0.1pM 到 1nM 的线性动态范围和~30fM 的检测限。
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