Institute of Biochemistry & Physiology of Plants & Microorganisms RAS, 13 Prospect Enthusiastic, Saratov 410049, Russia; Saratov State Agrarian University, Saratov 410012, Russia; Scientific Research Veterinary Institute, Saratov 410028, Russia.
Kotel'nikov Institute of Radio Engineering and Electronics RAS, Saratov Branch, Saratov 410019, Russia.
Talanta. 2018 Feb 1;178:569-576. doi: 10.1016/j.talanta.2017.09.076. Epub 2017 Sep 29.
The acoustic biological sensor for the analysis of the bacterial cells in conducting suspension was developed. The sensor represented the two channel delay line based on the piezoelectric plate of Y-X lithium niobate thick of 0.2mm. Two pairs of the interdigital transducers (IDT) for the excitation and reception of shear horizontal acoustic wave of zero order (SH) in each channel were deposited by the method of photolithography. One channel of the delay line was electrically shorted by the deposition of thin aluminum film between IDTs. The second channel remained as electrically open. The liquid container with the volume of 5ml was fixed on the plate surface between IDTs by the glue, which did not cause the additional insertion loss. For the first time the influence of the conductivity of the cell suspension on the registration of the specific and nonspecific interactions of the bacterial cells with phage-antibodies (phage-Abs) was studied by means of the developed sensor. The dependencies of the change in insertion loss and phase of the output signal on the conductivity of the buffer solution at specific/nonspecific interactions for the electrically open and shorted channels of the delay line were obtained. It was shown that the sensor successfully registered the interactions of microbial cells with phage-Abs in the range of the conductivity of 2-20 μS/cm on the model samples A. brasilense Sp245 - specific phage-Abs. The sensor in the time regime of the operation fast reacted on the specific/nonspecific interaction and the time of the stabilization of the output parameters did not exceed 10min.
研制了用于分析悬浮导电细菌细胞的声学生物传感器。该传感器代表了基于厚度为 0.2mm 的 Y-X 铌酸锂压电板的双通道延迟线。每个通道中的两对叉指换能器（IDT）用于激励和接收零阶剪切水平声波（SH）。通过光刻法沉积的薄铝薄膜将延迟线的一个通道电气短路。第二个通道保持电气开路。将体积为 5ml 的液体容器通过胶水固定在 IDT 之间的板表面上，这不会引起额外的插入损耗。首次利用所研制的传感器研究了细胞悬浮液电导率对细菌细胞与噬菌体-抗体（噬菌体-Abs）特异性和非特异性相互作用的检测影响。获得了延迟线开路和短路通道在特定/非特异性相互作用下，输出信号的插入损耗和相位随缓冲液电导率变化的关系。结果表明，该传感器在模型样品 A. brasilense Sp245-特异性噬菌体-Abs 的电导率为 2-20 μS/cm 的范围内成功地检测到微生物细胞与噬菌体-Abs 的相互作用。传感器在操作的时间域内快速响应于特异性/非特异性相互作用，并且输出参数的稳定时间不超过 10min。
