基于量子点的侧向流动免疫分析用于灵敏检测人血清中心脏脂肪酸结合蛋白（hFABP）。
A quantum dot-based lateral flow immunoassay for the sensitive detection of human heart fatty acid binding protein (hFABP) in human serum.
机构信息
Faculty of Chemistry, University of Bucharest, Regina Elisabeta Bd., No.4-12, Bucharest, Romania; Telemedica, Jean Louis Calderonno.28, Bucharest, Romania.
DDS Diagnostic, Vatra Luminoasa no.45, 031563 Bucharest, Romania.
出版信息
Talanta. 2018 Feb 1;178:910-915. doi: 10.1016/j.talanta.2017.10.045. Epub 2017 Oct 23.
We describe the preparation and validation of a novel lateral flow immunoassay test for the detection of human heart fatty acid binding protein (hFABP). Water-soluble CdTe quantum dots (QDs) were selected as the fluorescent label and were linked covalently to anti-hFABP antibodies. Upon conjugation, the secondary structure of the anti-hFABP was preserved and the fluorescence quantum yield of the CdTe QDs increased. The labelled antibodies were transferred to the immunoassay test strip and the antigen-antibody reaction was successfully performed. This evidenced the preserved antibody activity of QD-labelled anti-hFABP towards hFABP, and provided a rapid means for the quantitation of hFABP in human serum within the range of 0-160ng ∙ ml, with a much lower detection limit of 221pg.∙ ml compared with other rapid tests based on lateral flow immunoassays. This new immunoassay test has been successfully used for the early detection of acute myocardial infarction.
我们描述了一种新型侧向流动免疫分析检测方法的制备和验证，用于检测人心型脂肪酸结合蛋白（hFABP）。水溶性 CdTe 量子点（QDs）被选择作为荧光标记物，并与抗 hFABP 抗体共价连接。连接后，抗 hFABP 的二级结构得以保留，CdTe QDs 的荧光量子产率增加。标记的抗体被转移到免疫分析测试条上，并成功地进行了抗原-抗体反应。这证明了 QD 标记的抗 hFABP 对 hFABP 的抗体活性得到了保留，并提供了一种快速定量人血清中 hFABP 的方法，范围为 0-160ng ∙ ml，检测限低至 221pg ∙ ml，与其他基于侧向流动免疫分析的快速检测方法相比。这种新的免疫分析检测方法已成功用于急性心肌梗死的早期检测。
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