Faculty of Chemistry, University of Bucharest, Regina Elisabeta Bd., No.4-12, Bucharest, Romania; Telemedica, Jean Louis Calderonno.28, Bucharest, Romania.
DDS Diagnostic, Vatra Luminoasa no.45, 031563 Bucharest, Romania.
Talanta. 2018 Feb 1;178:910-915. doi: 10.1016/j.talanta.2017.10.045. Epub 2017 Oct 23.
We describe the preparation and validation of a novel lateral flow immunoassay test for the detection of human heart fatty acid binding protein (hFABP). Water-soluble CdTe quantum dots (QDs) were selected as the fluorescent label and were linked covalently to anti-hFABP antibodies. Upon conjugation, the secondary structure of the anti-hFABP was preserved and the fluorescence quantum yield of the CdTe QDs increased. The labelled antibodies were transferred to the immunoassay test strip and the antigen-antibody reaction was successfully performed. This evidenced the preserved antibody activity of QD-labelled anti-hFABP towards hFABP, and provided a rapid means for the quantitation of hFABP in human serum within the range of 0-160ng ∙ ml, with a much lower detection limit of 221pg.∙ ml compared with other rapid tests based on lateral flow immunoassays. This new immunoassay test has been successfully used for the early detection of acute myocardial infarction.
我们描述了一种新型侧向流动免疫分析检测方法的制备和验证，用于检测人心型脂肪酸结合蛋白（hFABP）。水溶性 CdTe 量子点（QDs）被选择作为荧光标记物，并与抗 hFABP 抗体共价连接。连接后，抗 hFABP 的二级结构得以保留，CdTe QDs 的荧光量子产率增加。标记的抗体被转移到免疫分析测试条上，并成功地进行了抗原-抗体反应。这证明了 QD 标记的抗 hFABP 对 hFABP 的抗体活性得到了保留，并提供了一种快速定量人血清中 hFABP 的方法，范围为 0-160ng ∙ ml，检测限低至 221pg ∙ ml，与其他基于侧向流动免疫分析的快速检测方法相比。这种新的免疫分析检测方法已成功用于急性心肌梗死的早期检测。
