Chicken shares an NFYB-regulated bidirectional promoter with a antisense transcript and inhibits cells proliferation and migration.

The chicken coiled-coil domain-containing protein 152 () recently has been identified as a novel one implicated in cell cycle regulation, cellular proliferation and migration by us. Here we demonstrate that is oriented in a head-to-head configuration with the antisense transcript of growth hormone receptor () gene. Through serial luciferase reporter assays, we firstly identified a minimal 102 bp intergenic region as a core bidirectional promoter to drive basal transcription in divergent orientations. And site mutation and transient transfected assays showed that nuclear transcription factor Y subunit beta (NFYB) could bind to the CCAAT box and directly transactivate this bidirectional promoter. SiRNA-mediated NFYB depletion could significantly down-regulate the expression of both and . Additionally, the expression of was significantly up-regulated after overexpression. Overexpression of remarkably reduced cell proliferation and migration through JAK2/STAT signaling pathway. Thus, the bidirectional transcription unit, as a novel direct target of NFYB, is possibly essential for the accelerated proliferation and motility of different cells.