Cagigal Elena Fernández-Miranda, Sánchez Abelardo Casares
Department of Biology of Organism and Systems, Area of Plant Physiology, University of Oviedo, 33006 Oviedo, Spain.
Mycobiology. 2017 Sep;45(3):172-177. doi: 10.5941/MYCO.2017.45.3.172. Epub 2017 Sep 30.
The genus is characterized by the difficulty of species identification, which results in reproducibility problems, as well as the need for large quantities of fungal inoculum. In particular, studies of have reported divergent results in the growth while little is known of its capacity to degrade organic matter. For all the above, and assuming that this variability could be due to an inappropriate culture media, the aim of this study was to analyse growth in different culture media (MMN, MS, and 1/2 MS) and in the case of MMN in presence/absence of two types of organic matter (fresh litter and senescence litter) to probe the saprophytic ability of . We also evaluated the effects of pH changes in the culture media. Growth kinetics was assessed by weekly quantification of the area of growth in solid culture media over 5 wk, calculating the growth curves and inflection points of each culture media. In addition, final biomass after 5 wk in the different culture media was calculated. Results showed that best culture media are MS and 1/2 MS. Moreover, an improvement in growth in culture media containing decomposing fall litter was observed, leading to confirm differences in the culture media of this species with others of the same genus. Further, we established that all growth media suffered a significant acidification after fungal growth.
该属的特点是物种鉴定困难,这导致了可重复性问题,以及需要大量的真菌接种物。特别是,对该属的研究报告了其在生长方面的不同结果,而对其降解有机物的能力却知之甚少。基于上述所有情况,并假设这种变异性可能是由于培养基不合适,本研究的目的是分析其在不同培养基(MMN、MS和1/2 MS)中的生长情况,对于MMN培养基,还要分析在存在/不存在两种有机物质(新鲜凋落物和衰老凋落物)的情况下其腐生能力。我们还评估了培养基pH值变化的影响。通过每周对固体培养基中5周内的生长面积进行量化来评估生长动力学,计算每种培养基的生长曲线和拐点。此外,还计算了在不同培养基中培养5周后的最终生物量。结果表明,最佳培养基是MS和1/2 MS。此外,观察到含有分解秋季凋落物的培养基中生长有所改善,这证实了该物种与同属其他物种在培养基方面存在差异。此外,我们还确定,真菌生长后所有生长培养基都出现了显著酸化。